Combining chemistry and protein engineering for new-to-nature biocatalysis.
Combining chemistry and protein engineering for new-to-nature biocatalysis.
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DOI：
10.1038/s44160-021-00008-x
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Arnold FH
中科院分区：
文献类型：
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作者：
Miller DC;Athavale SV;Arnold FH
生物催化是应用酶来解决人类需要的合成问题，已经发展成为一项强有力的化学创新技术。在过去的十年中，自然界提供了酶催化的蓝图，化学家引入了具有非生物底物的创新活性模式，蛋白质工程师开发了新的工具和算法来调整和改进酶的功能，这三方面的合作伙伴关系揭开了新自然酶催化的前沿。从这个角度来看，我们强调了跨学科研究的例子，这些研究有助于扩大生物催化的范围，包括通过仿生学的视角探索酶的多功能性概念，以实现目前化学催化不可能实现的活性和选择性。我们指出现代工具，如定向进化，计算蛋白质设计和基于机器学习的蛋白质工程方法，已经影响并将继续影响酶工程的新非生物转化。预计在未来几年，跨学科的持续合作将推动生物催化的进一步发展。
Biocatalysis, the application of enzymes to solve synthetic problems of human import, has blossomed into a powerful technology for chemical innovation. In the past decade, a threefold partnership, where nature provides blueprints for enzymatic catalysis, chemists introduce innovative activity modes with abiological substrates, and protein engineers develop new tools and algorithms to tune and improve enzymatic function, has unveiled the frontier of new-to-nature enzyme catalysis. In this perspective, we highlight examples of interdisciplinary studies which have helped to expand the scope of biocatalysis, including concepts of enzymatic versatility explored through the lens of biomimicry, to achieve both activities and selectivities that are not currently possible with chemocatalysis. We indicate how modern tools, such as directed evolution, computational protein design and machine learning-based protein engineering methods, have already impacted and will continue to influence enzyme engineering for new abiological transformations. A sustained collaborative effort across disciplines is anticipated to spur further advances in biocatalysis in the coming years.