The nuclear protein expression levels of SNAI1 and ZEB1 are involved in the progression and lymph node metastasis of cervical cancer via the epithelial-mesenchymal transition pathway
The nuclear protein expression levels of SNAI1 and ZEB1 are involved in the progression and lymph node metastasis of cervical cancer via the epithelial-mesenchymal transition pathway
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DOI：
10.1016/j.humpath.2013.04.001
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发表时间：
2013-10-01
期刊：
影响因子：
3.3
通讯作者：
Wang, Shixuan
中科院分区：
文献类型：
--
作者：
Chen, Zhilan;Li, Shuang;Wang, Shixuan
越来越多的证据表明，上皮-间质转化的异常激活在肿瘤细胞的侵袭和转移中起着关键作用。转录因子SNAI 1和ZEB 1调节上皮-间充质转化。为了确定SNAI 1和ZEB 1是否参与宫颈癌的转移，我们使用免疫组织化学来评估SNAI 1、ZEB 1和波形蛋白在大量宫颈癌样本的肿瘤和间质隔室中的表达。使用H评分（百分比x强度）评价结果。在70例乳腺癌组织中，64例（91.43%）SNAI 1表达阳性。中位SNAI 1 H评分为174.00（范围：5-285）。67例（95.71%）ZEB 1阳性，中位H评分为165.77（范围，5-260）。肿瘤细胞中SNAI 1和ZEB 1的核表达与国际妇产科联合会（FIGO）分期（分别为P = .015和P = .008）和淋巴结转移（分别为P = .007和P = .007）呈正相关;同时，肿瘤细胞中波形蛋白的表达与淋巴结转移呈正相关（P = .019）。根据阴性波形蛋白表达，肿瘤细胞中ZEB 1的核表达与FIGO分期呈正相关（P = 0.04）。根据阳性波形蛋白表达，肿瘤细胞中SNAI 1的核表达与FIGO分期（P = 0.018）和pN（P = 0.029）呈正相关。根据这些发现，我们提出SNAI 1和ZEB 1有可能被用作盆腔淋巴结转移的新预测因子，并代表宫颈癌患者有希望的治疗靶点。(C)2013 Elsevier Inc. All rights reserved.
Growing evidence illustrates that aberrant activation of the epithelial-mesenchymal transition plays a key role in tumor cell invasion and metastasis. Transcription factors SNAI1 and ZEB1 regulate the epithelial-mesenchymal transition. To determine if SNAI1 and ZEB1 are involved in the metastasis of cervical cancer, we used immunohistochemistry to evaluate the expression of SNAIL ZEB1, and vimentin in tumor and stromal compartments for a large set of cervical carcinoma samples. Results were evaluated using an H score (percentage x intensity). Of 70 samples, 64 cases (91.43%) were positive for SNAI1 expression. The median SNAI1 H score was 174.00 (range, 5-285). Sixty-seven cases (95.71%) were positive for ZEB1, with a median H score of 165.77 (range, 5-260). Nuclear expression of SNAI1 and ZEB1 in tumor cells was positively associated with International Federation of Gynecology and Obstetrics (FIGO) stages (P = .015 and P = .008, respectively) and lymph node metastasis (P = .007 and P = .007, respectively); meanwhile, expression of vimentin in tumor cells was positively associated with lymph node metastasis (P = .019). According to negative vimentin expression, nuclear expression of ZEB1 in tumor cells was positively associated with FIGO stages (P = .04). According to positive vimentin expression, nuclear expression of SNAI1 in tumor cells was positively associated with FIGO stages (P = .018) and pN (P = .029). In light of these findings, we propose that SNAI1 and ZEB1 have the potential to be used as a novel predictor of pelvic lymph node metastasis and represent promising therapeutic targets in patients with cervical cancer. (C) 2013 Elsevier Inc. All rights reserved.