The F-box E3 ubiquitin ligase AtSDR is involved in salt and drought stress responses in Arabidopsis.
The F-box E3 ubiquitin ligase AtSDR is involved in salt and drought stress responses in Arabidopsis.
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DOI：
10.1016/j.gene.2021.146011
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发表时间：
2021-10
期刊：
影响因子：
3.5
通讯作者：
B. Li;Shuai Gao;Zhimin Yang;Jianbo Song
中科院分区：
文献类型：
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作者：
B. Li;Shuai Gao;Zhimin Yang;Jianbo Song
F-box蛋白基因在植物发育和逆境应答中起着重要作用。在拟南芥中，有超过600个F-box蛋白，它们的功能大多不清楚。本研究表明F-box（SKP 1-Cullin/CDC 53-F-box）基因At 5g 15710（SaltandDroughResponsiveness，SDR）参与拟南芥的非生物胁迫反应，SDR在拟南芥的所有组织中均有表达，并且在盐、热胁迫和阿坝处理下表达上调，而在干旱胁迫下表达下调。亚细胞定位分析表明SDR蛋白与细胞核共定位。35 S：AntiSDR植物对盐胁迫高度敏感，而35 S：SDR植物表现出耐盐表型。此外，35 S：SDR植物对干旱胁迫高度敏感，而35 S：AntiSDR植物则显著更耐旱。总体而言，我们的结果表明SDR参与了拟南芥的盐和干旱胁迫反应。
F-box protein genes have been shown to play vital roles in plant development and stress respones. InArabidopsis, there are more than 600 F-box proteins, and most of their functions are unclear. The present study shows that the F-box (SKP1-Cullin/CDC53-F-box) gene At5g15710 (SaltandDrought Responsiveness,SDR) is involved in abiotic stress responses inArabidopsis.SDRis expressed in all tissues ofArabidopsisand is upregulated by salt and heat stresses and ABA treatment but downregulated by drought stress. Subcellular localization analysis shows that the SDR protein colocalizes with the nucleus.35S:AntiSDRplants are hypersensitive to salt stress, but35S:SDRplants display a salt-tolerant phenotype. Furthermore,35S:SDRplants are hypersensitive to drought stress, while35S:AntiSDRplants are significantly more drought tolerant. Overall, our results suggest that SDR is involved in salt and drought stress responses inArabidopsis.