IN-VITRO AND IN-VIVO TUMORICIDAL PROPERTIES OF A PATHOGENIC FREE-LIVING AMEBA
IN-VITRO AND IN-VIVO TUMORICIDAL PROPERTIES OF A PATHOGENIC FREE-LIVING AMEBA
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DOI：
10.1016/0304-3835(93)90016-3
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发表时间：
1993-08-16
期刊：
影响因子：
9.7
通讯作者：
NIEDERKORN, JY
中科院分区：
文献类型：
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作者：
PIDHERNEY, MS;ALIZADEH, H;NIEDERKORN, JY
在体内和体外研究了致病/自由生活的卡氏阿米巴的趋化和杀肿瘤特性。A. Castellanii滋养体对人黑色素瘤（OCM-1）和鼠黑色素瘤（D5.1G4）细胞显示强阳性趋化反应。虽然寄生虫通常侵入并破坏角膜上皮和基质，但对这些角膜成分的提取物均未检测到阳性趋化反应。体外研究表明，活的寄生虫，以及无细胞寄生虫裂解物，产生迅速和广泛的多种肿瘤细胞的细胞溶解。A. Castellanii进行了体内检查，并揭示了将活寄生虫或无细胞寄生虫裂解物注射到进行性生长的皮下黑素瘤中，与未处理的对照相比，导致肿瘤质量减少83%和53%。利用致病性/自由生活阿米巴及其无细胞产物的杀肿瘤特性治疗耐药或耐辐射肿瘤的可行性值得进一步研究。
The chemotactic and tumoricidal properties of the pathogenic/free-living amoeba Acanthamoeba castellanii were examined in vivo and in vitro. A. castellanii trophozoites displayed strong positive chemotactic responses to human melanoma (OCM-1) and murine melanoma (D5.1G4) cells. Although the parasites typically invade and destroy the corneal epithelium and stroma, positive chemotactic responses were not detected against extracts of either of these corneal elements. In vitro studies revealed that viable parasites, as well as cell-free parasite lysates, produced swift and extensive cytolysis of a wide variety of tumor cells. The tumoricidal properties of A. castellanii were examined in vivo and revealed that injection of viable parasites or cell-free parasite lysates into progressively growing subcutaneous melanomas resulted in 83% and 53% reductions in the tumor masses compared with untreated controls. The feasibility of utilizing the tumoricidal properties of pathogenic/free-living amoebae and their cell-free products in the treatment of drug-resistant or radioresistant tumors warrants further investigation.