Transcriptome analysis of wheat grain using RNA-Seq
Transcriptome analysis of wheat grain using RNA-Seq
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DOI：
10.15302/j-fase-2014024
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Baoyun Li
中科院分区：
文献类型：
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作者：
Liu Wei;Zhihui Wu;Yufeng Zhang;Dandan Guo;Yuzhou Xu;Weixia Chen;Haiying Zhou;Mingshan You;Baoyun Li
With the increase in consumer demand, wheat.grain quality improvement has become a focus in China.and worldwide. Transcriptome analysis is a powerful.approach to research grain traits and elucidate their genetic.regulation. In this study, two cDNA libraries from the.developing grain and leaf-stem components of bread wheat.cultivar, Nongda211, were sequenced using Roche/454.technology. There were 1061274 and 1516564 clean reads.generated from grain and leaf-stem, respectively. A total of.61393 high-quality unigenes were obtained with an.average length of 1456 bp after de novo assembly. The.analysis of the 61393 unigenes involved in the biological.processes of the grain showed that there were 7355.differentially expressed genes upregulated in the grain.library. Gene ontology enrichment and the Kyoto Ency-.clopedia of Genes and Genomes pathway enrichment.analysis showed that many transcription products and.transcription factors associated with carbohydrate and.protein metabolism were abundantly expressed in the.grain. These results contribute to excavategenes associated.with wheat quality and further study how they interact.
