Central amylin expression and its induction in rat dams
Central amylin expression and its induction in rat dams
复制标题
DOI：
10.1111/j.1471-4159.2009.06422.x
复制
发表时间：
2009-12-01
影响因子：
4.7
通讯作者：
Dobolyi, Arpad
中科院分区：
文献类型：
--
作者：
Dobolyi, Arpad
胰淀素或胰岛淀粉样多肽被认为是在胰腺β细胞中表达而不是在大脑中表达的饱足信号。本研究探讨产后下丘脑视前区mRNA表达的调控。在微阵列实验中比较了哺乳期母鼠和分娩后立即取出幼崽的母鼠的mRNA水平。产后9天，有20个基因的表达显著增加，14个基因的表达减少。胰淀素mRNA水平表现出最大的变化，增加了25.7倍。定量RT-PCR测量验证了泌乳期母鼠视前区胰淀素mRNA水平的增加，而降钙素基因相关肽家族其他成员的表达水平没有变化。胰淀素的原位杂交组织化学进一步验证了其在哺乳期母亲的诱导，并证明了胰淀素mRNA的分布在内侧视前核，部分内侧视前区，和腹侧部分的床核的终纹，但在其他地方在大鼠大脑。免疫标记证实产后诱导胰淀素在视前区的肽水平，以及。结果表明，胰淀素可能在母体调节中发挥作用。
Amylin, or islet amyloid polypeptide, is known as a satiating signal expressed in pancreatic beta-cells but not in the brain. In this study, regulations of postpartum mRNA expressions were investigated in the preoptic area of the hypothalamus. mRNA levels of lactating dams and mothers whose pups were removed immediately after delivery were compared in microarray experiments. There were 20 genes identified with significantly increased and 14 with decreased expression 9 days postpartum. Amylin mRNA level demonstrated the largest change, a 25.7 times increase. Quantitative RT-PCR measurements validated the increase in the mRNA level of amylin in the preoptic area of lactating dams while the expression level of other members of the calcitonin gene-related peptide family did not change. In situ hybridization histochemistry for amylin further verified its induction in lactating mothers and demonstrated the distribution of amylin mRNA in the medial preoptic nucleus, parts of the medial preoptic area, and the ventral part of the bed nucleus of the stria terminalis but nowhere else in the rat brain. Immunolabeling verified the postpartum induction of amylin in the preoptic area at the peptide level, as well. The results suggest that amylin may play a part in maternal regulations.