The FHY3 and FAR1 genes encode transposase-related proteins involved in regulation of gene expression by the phytochrome A-signaling pathway
The FHY3 and FAR1 genes encode transposase-related proteins involved in regulation of gene expression by the phytochrome A-signaling pathway
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DOI：
10.1046/j.1365-313x.2003.01741.x
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发表时间：
2003-05-01
期刊：
影响因子：
7.2
通讯作者：
Quail, PH
中科院分区：
文献类型：
--
作者：
Hudson, ME;Lisch, DR;Quail, PH
拟南芥突变体far 1和fhy 3显示出下胚轴伸长抑制减少的表型，这是特异性的远红光，因此特异性的光敏色素A（phyA）信号通路。我们报告FAR 1和FHY 3基因编码的蛋白质都与II型MuDR家族转座子的转座酶相关。我们证明，FAR 1蛋白能够激活拟南芥中的转录，表明它可能定义一种类型的转录调节因子。使用微阵列表达分析，我们表明，在野生型Col-0植物的连续远红光诱导的293个mRNA的两倍，85%的fhy 3突变体的反应性降低。显著的改变，观察到的响应的基因编码的某些转录因子，蛋白质参与细胞壁的延伸，和蛋白质相关的氧化还原平衡控制。我们还发现了一些基因，包括一些参与转录控制的基因，这些基因在黑暗生长的突变植物中表现出改变的转录行为。两者合计，我们的数据表明，FAR 1和FHY 3可能发挥作用的光调控发展的信号转导途径以外的“许可”，但需要转录调控成分的表达。另一种可能性是，它们的作用包括光信号转导和对光无反应的其他基因的转录调节。我们认为FAR 1和FHY 3通过一种机制控制其靶基因的表达，该机制直接从祖先MuDRA样转座酶与移动的元件的TIR结合的方式进化而来。
The Arabidopsis mutants far1 and fhy3 display a phenotype of reduced inhibition of hypocotyl elongation, which is specific to far-red light and therefore specific to the phytochrome A (phyA)-signaling pathway. We report that the proteins encoded by the FAR1 and FHY3 genes are both related to the transposases of type II MuDR family transposons. We demonstrate that the FAR1 protein is capable of activating transcription in Arabidopsis , indicating that it may define a type of transcriptional regulator. Using microarray expression analysis, we show that of 293 mRNAs twofold induced in wild-type Col-0 plants by continuous far-red light, 85% show reduced responsiveness in the fhy3 mutant. Notable alterations were observed in the responses of genes encoding certain transcription factors, proteins involved in cell wall extension, and proteins related to redox balance control. We also found genes, including some involved in transcriptional control, which showed altered transcriptional behavior in the dark-grown mutant plants. Taken together, our data suggest that FAR1 and FHY3 may function 'permissively' outside the signal transduction pathway of light-regulated development, yet be required for the expression of transcriptional regulatory components. An alternative possibility is that their role includes both light-signal transduction and transcriptional regulation of other genes not responsive to light. We propose that FAR1 and FHY3 control the expression of their target genes by a mechanism that has evolved directly from the way that an ancestral, MuDRA-like transposase bound to the TIRs of mobile elements.