An immunisation strategy for the protection of cattle against alcelaphine herpesvirus-1-induced malignant catarrhal fever
An immunisation strategy for the protection of cattle against alcelaphine herpesvirus-1-induced malignant catarrhal fever
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DOI：
10.1016/j.vaccine.2008.06.056
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发表时间：
2008-08-18
期刊：
影响因子：
5.5
通讯作者：
Russell, George C.
中科院分区：
文献类型：
--
作者：
Haig, David M.;Grant, Dawn;Russell, George C.
本研究的目的是刺激牛的口-鼻-咽区域的免疫力，以保护它们免受阿卡拉芬疱疹病毒-1（AlHV-1）诱导的恶性卡他热。将减毒的C500菌株AlHV-1与弗氏佐剂一起沿着肌内（IM）用于上颈部区域以免疫牛。将毒力C500菌株AlHV-1用于鼻内攻击。十头牛中有九头受到保护。保护作用与鼻分泌物中高水平的中和抗体有关。攻毒后，一些受保护动物的血液样本和一个淋巴结样本中显示出一过性低水平的病毒DNA，而在所有MCF动物的血液和淋巴结样本中均检测到病毒DNA。这是迄今为止控制恶性卡他热最有希望的免疫策略。(C)2008爱思唯尔有限公司保留所有权利。
The aim of this study was to stimulate immunity in the oro-nasal-pharyngeal region of cattle to protect them from alcelaphine herpesvirus-1 (AlHV-1)-induced malignant catarrhal fever. Attenuated C500 strain AlHV-1 was used along with Freund's adjuvant intramuscularly (IM) in the upper neck region to immunise cattle. Virulent C500 strain AlHV-1 was used for intranasal challenge. Nine of ten cattle were protected. Protection was associated with high levels of neutralising antibody in nasal secretions. Some protected animals showed transient low levels of viral DNA in blood samples and in one lymph node sample after challenge whereas viral DNA was detected in the blood and in lymph node samples of all animals with MCF. This is the most promising immunisation strategy to date for the control of malignant catarrhal fever. (C) 2008 Elsevier Ltd. All rights reserved.