Effects of microbeam radiation therapy on normal and tumoral blood vessels
Effects of microbeam radiation therapy on normal and tumoral blood vessels
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DOI：
10.1016/j.ejmp.2015.04.014
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发表时间：
2015-09-01
影响因子：
3.4
通讯作者：
Djonov, Valentin
中科院分区：
文献类型：
--
作者：
Bouchet, Audrey;Serduc, Raphaeel;Djonov, Valentin
微束放射治疗(MRT)是一种新的临床前放射治疗形式，它使用准平行的同步X射线微束阵列。虽然在微束路径上沉积了数百个灰质，但正常脑组织表现出高度的耐受性，并伴随着表面上正常的血管的持久性。相反，MRT控制肿瘤生长的效率被认为与优先破坏肿瘤血管有关。体内双光子显微镜、磁共振成像和组织学研究表明，在不同的动物模型中，健康血管网络的高阻力被证明。虽然通透性出现了短暂的增加，但血管的结构保持完好。使用不同发育阶段的鸡绒毛膜尿囊膜表明，微束引起的损伤依赖于血管的成熟。体内和超微结构观察显示，微束对发育后期成熟的血管系统的影响可以忽略不计；然而，微束路径中未成熟的毛细血管丛被完全破坏。MRT在啮齿动物模型中的应用显示了对肿瘤血管的优先作用。虽然在正常脑组织的血管系统中没有观察到重大的改变，但肿瘤表现为毛细血管剥蚀，伴随着一过性的通透性增加，然后是肿瘤的灌注量减少，最后是肿瘤血管的数量减少。因此，MRT是一种非常有前景的治疗策略，其显著的肿瘤控制作用很可能是基于MRT的抗血管作用。(C)2015年，由爱思唯尔有限公司代表意大利医学协会出版。这是一篇CC BY-NC-ND许可证下的开放获取文章。
Microbeam radiation therapy (MRT) is a new form of preclinical radiotherapy using quasi-parallel arrays of synchrotron X-ray microbeams. While the deposition of several hundred Grays in the microbeam paths, the normal brain tissues presents a high tolerance which is accompanied by the permanence of apparently normal vessels. Conversely, the efficiency of MRT on tumor growth control is thought to be related to a preferential damaging of tumor blood vessels.The high resistance of the healthy vascular network was demonstrated in different animal models by in vivo biphoton microscopy, magnetic resonance imaging, and histological studies. While a transient increase in permeability was shown, the structure of the vessels remained intact. The use of a chick chorioallantoic membrane at different stages of development showed that the damages induced by microbeams depend on vessel maturation. In vivo and ultrastructural observations showed negligible effects of microbeams on the mature vasculature at late stages of development; nevertheless a complete destruction of the immature capillary plexus was found in the microbeam paths. The use of MRT in rodent models revealed a preferential effect on tumor vessels. Although no major modification was observed in the vasculature of normal brain tissue, tumors showed a denudation of capillaries accompanied by transient increased permeability followed by reduced tumor perfusion and finally, a decrease in number of tumor vessels. Thus, MRT is a very promising treatment strategy with pronounced tumor control effects most likely based on the anti-vascular effects of MRT. (C) 2015 Published by Elsevier Ltd on behalf of Associazione Italiana di Fisica Medica. This is an open access article under the CC BY-NC-ND license.