Model Phospholipid Liposomes to Study the β-Amyloid-Peptide-Induced Membrane Disruption.
Model Phospholipid Liposomes to Study the β-Amyloid-Peptide-Induced Membrane Disruption.
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DOI：
10.1007/978-1-4939-7811-3_23
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Doherty KE
中科院分区：
文献类型：
--
作者：
Qiang W;Doherty KE
模型磷脂脂质体已被广泛用于研究多肽与膜双层之间的分子相互作用。在阿尔茨海默病（Alzheimer's disease，AD）发病机制的研究中，β-淀粉样蛋白（β-amyloid，Aβ）多肽对神经细胞膜的破坏被认为是其神经毒性的主要原因。然而，由于多种途径的共存，在高分辨率水平上清楚地解释Aβ诱导的细胞膜是具有挑战性的。在这里，我们提出了简化模型脂质体系统的生成，这将有利于深入的机制研究。将描述模型脂质体的制备和单个膜破坏效应的表征的方案。
Model phospholipid liposomes have been utilized widely to study the molecular interactions between peptides and membrane bilayers. In the mechanistic study of Alzheimer’s disease (AD), disruption of neuronal cell membranes has been considered as a major contribution for the β-amyloid (Aβ) peptides’ neurotoxicity. However, clear interpretation of the Aβ-induced cellular membrane at high-resolution level is challenging because of the co-existence of multiple pathways. Here we present the generation of simplified model liposome systems that will facilitate the in-depth mechanistic studies. Protocols for the preparation of model liposomes and the characterization of individual membrane disruption effects will be described.