Sex matters: XIST and DDX3Y gene expression as a tool to determine fetal sex in human first trimester placenta
Sex matters: XIST and DDX3Y gene expression as a tool to determine fetal sex in human first trimester placenta
复制标题
DOI：
10.1016/j.placenta.2020.06.016
复制
发表时间：
2020-08-01
期刊：
影响因子：
3.8
通讯作者：
Majali-Martinez, Alejandro
中科院分区：
文献类型：
--
作者：
Hoch, Denise;Novakovic, Boris;Majali-Martinez, Alejandro
胎儿性别影响胎盘功能以及母婴健康，是妊娠研究中需要考虑的重要因素。然而，妊娠前三个月的胎儿性别测定仍面临一些技术限制。在这里，我们描述了一种 RT-qPCR 技术，根据 X 失活特异性转录本 (XIST) 和 DEAD-Box 解旋酶 3 Y 连锁 (DDX3Y) 基因表达来确定胎儿性别。该方法简单、可靠、快速且适用于胎盘组织和原代细胞。
Fetal sex influences placental function as well as maternal and fetal health, being an important factor to consider in pregnancy studies. However, fetal sex determination in the first trimester of pregnancy still faces some technical limitations. Here we describe an RT-qPCR technique to determine fetal sex based on X-inactive specific transcript (XIST) and DEAD-Box helicase 3 Y-linked (DDX3Y) gene expression. This method is straightforward, reliable, fast and applicable on both, placental tissue and primary cells.