Online respiration activity measurement (OTR, CTR, RQ) in shake flasks
Online respiration activity measurement (OTR, CTR, RQ) in shake flasks
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DOI：
10.1016/s1369-703x(03)00181-5
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发表时间：
2004-03-01
影响因子：
3.9
通讯作者：
Büchs, J
中科院分区：
文献类型：
--
作者：
Anderlei, T;Zang, W;Büchs, J
用废气分析在线测量搅拌生物反应器中微生物培养物的呼吸活性(包括氧转移速率(OTR)、二氧化碳转移速率(CTR)、呼吸商(RQ))多年来一直是最先进的方法。由于与搅拌生物反应器相比，在摇动生物反应器中进行了更多的实验，Anderlei和Buchs[Biochem]。英语。J.7(2001)157]开发了一种在无菌条件下在线测定摇瓶中氧气转移率的测量装置(OTR-装置)。本文介绍了一种扩展的OTR装置，称为呼吸活动监测系统(RAMOS)，它允许在摇动生物反应器中额外测量二氧化碳转移率和呼吸商。本文介绍了用拉莫斯发酵酿酒酵母和树干毕赤酵母的情况。这些测量非常清楚地显示了CRABTREE阳性的酿酒酵母和CRABTREE阴性的酵母P stipitis之间的呼吸活性的差异。此外，还介绍了谷氨酸棒杆菌的发酵，说明了氧气限制对培养物代谢活动的影响。此外，对杂交瘤细胞系进行了Ramos发酵，以阐明该系统的测量灵敏度。新设备提供了代表在线生物培养的最重要和最具特征的参数(OTR、CTR、RQ)，使用户能够对摇动生物反应器中已经存在的微生物的新陈代谢得出结论。(C)2003爱思唯尔B.V.保留所有权利。
Online measurement of respiration activity (including oxygen transfer rate (OTR), carbon dioxide transfer rate (CTR), respiratory quotient (RQ)) of microbial cultures in stirred bioreactors with exhaust gas analysis has been state of the art for years. As much more experiments are conducted in shaking bioreactors compared to stirred bioreactors, Anderlei and Buchs [Biochem. Eng. J. 7 (2001) 157] developed a measuring device (OTR-Device) for online determination of the oxygen transfer rate in shake flasks under sterile conditions. In this paper, an extension of the OTR-Device, termed respiration activity monitoring system (RAMOS) is described, which allows additional measurement of the carbon dioxide transfer rate and the respiratory quotient in shaking bioreactors. Fermentations of the yeasts Saccharomyces cerevisiae and Pichia stipitis carried out with RAMOS are presented. These measurements show very clearly the differences in respiration activities between the Crabtree-positive yeast S. cerevisiae and the Crabtree-negative yeast P stipitis. Furthermore, a fermentation of the bacterium Corynebacterium glutamicum is presented, showing the influence of an oxygen limitation on the metabolic activities of the culture. Also, a fermentation of a hybridoma cell line was carried out with RAMOS to elucidate the measuring sensitivity of the system. The new device provides the most important and characteristic parameters (OTR, CTR, RQ) representing biological cultures online, enabling users to draw conclusions on metabolisms of microorganisms already in shaking bioreactors. (C) 2003 Elsevier B.V. All rights reserved.