The protein kinase encoded in the short unique region of pseudorabies virus: description of the gene and identification of its product in virions and in infected cells.
The protein kinase encoded in the short unique region of pseudorabies virus: description of the gene and identification of its product in virions and in infected cells.
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伪狂犬病病毒短独特区域编码的蛋白激酶：该基因的描述及其在病毒体和感染细胞中产物的鉴定。
DOI：
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发表时间：
1990
影响因子：
3.8
通讯作者：
D. Leader
中科院分区：
文献类型：
--
作者：
G. Zhang;R. Stevens;D. Leader
我们克隆并确定了一个基因pk的核苷酸序列，该基因位于伪狂犬病毒（PRV）短独特区域编码糖蛋白X基因的上游。该基因有可能编码334个氨基酸的蛋白质，并且与1型单纯疱疹病毒（HSV-1）的基因US3相关，该基因已被证明编码一种蛋白激酶。预测PRV pk基因编码的氨基酸序列与HSV-1 US3基因编码的相应序列在c端催化域同源，但在n端催化域存在明显差异。与HSV-1一样，pk基因的mRNA似乎与糖蛋白下游的mRNA位于3'共端。抗血清针对PRV pk部分催化结构域与大肠杆菌β -半乳糖苷酶融合产生的蛋白。这与先前描述的物理均质蛋白激酶PRV- pk特异性反应，PRV- pk是从裂解感染PRV的仓鼠成纤维细胞中分离出来的。虽然大多数PRV- pk存在于细胞质中，但使用相同的抗体在纯化的PRV病毒粒子中也检测到一些；使用针对相应的HSV-1融合蛋白的抗血清，发现了类似的HSV-1蛋白激酶分布。当与热灭活的病毒粒子一起呈现时，纯化的PRV-PK（与病毒粒子中也存在的某些细胞蛋白激酶一样）能够在体外磷酸化体内磷酸化的主要病毒粒子的磷酸化蛋白。
We have cloned and determined the nucleotide sequence of a gene, pk, that lies immediately upstream from the gene encoding glycoprotein X in the short unique region of the alphaherpesvirus, pseudorabies virus (PRV). The gene has the potential to encode a protein of 334 amino acids, and is related to gene US3 of herpes simplex virus type 1 (HSV-1), which has been shown to encode a protein kinase. The predicted amino acid sequence encoded by the PRV pk gene is homologous to the corresponding sequence encoded by the HSV-1 US3 gene in the C-terminal catalytic domain, but diverges markedly in the N-terminal domain. As with HSV-1, the mRNA for the pk gene appears to be 3' coterminal with that for the glycoprotein downstream. An antiserum was raised against a protein generated from the fusion of part of the PRV pk catalytic domain with Escherichia coli beta-galactosidase. This specifically reacted with a previously described physically homogeneous protein kinase, PRV-PK, isolated from hamster fibroblasts lytically infected with PRV. Although the majority of the PRV-PK is found in the cytoplasm, some was also detected in purified PRV virions by using the same antibody; a similar distribution was found for the HSV-1 protein kinase, using an antiserum raised against the corresponding HSV-1 fusion protein. When presented with heatinactivated virions, purified PRV-PK (in common with certain cellular protein kinases also present in the virion) was able to phosphorylate in vitro the major virion phosphoprotein phosphorylated in vivo.
DOI：
10.1073/pnas.85.10.3377
发表时间：
1988-05-01
影响因子：
11.1
作者：
JONES, SW;ERIKSON, E;ERIKSON, RL
通讯作者：
ERIKSON, RL