Proteomic analysis of cold stress-responsive proteins in Thellungiella rosette leaves
Proteomic analysis of cold stress-responsive proteins in Thellungiella rosette leaves
复制标题
丝藻莲座叶中冷应激反应蛋白的蛋白质组学分析
DOI：
10.1007/s00425-009-1003-6
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发表时间：
2009-10-01
期刊：
影响因子：
4.3
通讯作者：
Zhang, Genfa
中科院分区：
文献类型：
--
作者：
Gao, Fei;Zhou, Yijun;Zhang, Genfa
低温是影响植物生长和农业生产的最严重的环境因素之一。为了研究拟南芥样嗜盐芥（Thellungiella halophila）对冷胁迫的适应性，采用基于双向电泳的比较蛋白质组学方法，鉴定了Thellungiella莲座叶片在短时间（6小时、2天和5天）和长时间（24天）低温胁迫下丰度变化的蛋白质。66个蛋白点至少在一个时间点发生显著变化，长期冷应激组蛋白组变化最大，6 h冷处理组变化最小。质谱分析鉴定了50个蛋白点。鉴定的蛋白质主要参与光合作用、RNA代谢、防御反应、能量途径、蛋白质合成、折叠和降解、细胞壁和细胞骨架以及信号转导。这些蛋白可能协同工作，在冷胁迫下建立新的体内平衡。近一半已鉴定的冷响应蛋白与叶绿体生理的各个方面有关，这表明嗜盐T. halophila的冷胁迫耐受性至少部分是通过叶绿体功能的调节实现的。在低温处理下，所有参与RNA代谢、防御反应、蛋白质合成、折叠和降解的蛋白位点均显著上调，表明RNA代谢、防御和蛋白质代谢的增强可能在嗜盐酵母耐冷机制中起重要作用。
Low temperature is one of the most severe environmental factors that impair plant growth and agricultural production. To investigate how Thellungiella halophila, an Arabidopsis-like extremophile, adapts to cold stress, a comparative proteomic approach based on two-dimensional electrophoresis was adopted to identify proteins that changed in abundance in Thellungiella rosette leaves during short term (6 h, 2 and 5 days) and long term (24 days) exposure to cold stress. Sixty-six protein spots exhibited significant change at least at one time point and maximal cold stress induced-proteome change was found in long-term cold stress group while the minimal change was found in 6-h cold treatment group. Fifty protein spots were identified by mass spectrometry analysis. The identified proteins mainly participate in photosynthesis, RNA metabolism, defense response, energy pathway, protein synthesis, folding and degradation, cell wall and cytoskeleton and signal transduction. These proteins might work cooperatively to establish a new homeostasis under cold stress. Nearly half of the identified cold-responsive proteins were associated with various aspects of chloroplast physiology suggesting that the cold stress tolerance of T. halophila is achieved, at least partly, by regulation of chloroplast function. All protein spots involved in RNA metabolism, defense response, protein synthesis, folding and degradation were found to be upregulated markedly by cold treatment, indicating enhanced RNA metabolism, defense and protein metabolism may play crucial roles in cold tolerance mechanism in T. halophila.