Arabidopsis Class II TCP Transcription Factors Integrate with the FT-FD Module to Control Flowering
Arabidopsis Class II TCP Transcription Factors Integrate with the FT-FD Module to Control Flowering
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DOI：
10.1104/pp.19.00252
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发表时间：
2019-09-01
期刊：
影响因子：
7.4
通讯作者：
Yu, Diqiu
中科院分区：
文献类型：
--
作者：
Li, Daibo;Zhang, Haiyan;Yu, Diqiu
适当的开花时间对植物繁殖成功至关重要。虽然FLOWERLOCUS T（FT）-FD模块在光周期开花途径中起着至关重要的作用，但涉及的潜在机制和信号通路仍然不清楚。在这里，我们证明了II类TCP转录因子（TF）整合到FT-FD复合物中控制拟南芥（Arabidopsis thaliana）的成花起始。II类肉桂（CIN）TCP TF通过直接结合下游花分生组织身份基因（如APETALA 1（AP 1））的启动子而起转录激活剂的作用。此外，这些TCP直接与FD相互作用，FD是一种基本的Leu拉链TF，在光周期开花中起着关键作用，进一步激活AP 1的表达。遗传分析表明，II类CIN TCP TF与FT和FD协同作用，以AP 1依赖的方式正向调节开花。因此，我们的研究结果提供了令人信服的证据表明，II类CIN TCP TF直接作用于AP 1启动子，以增强其转录，从而进一步阐明了拟南芥光周期开花调控的分子机制。
The appropriate timing of flowering is critical for plant reproductive success. Although the FLOWERING LOCUS T (FT)-FD module plays crucial roles in the photoperiodic flowering pathway, the underlying mechanisms and signaling pathways involved still remain elusive. Here, we demonstrate that class II TCP transcription factors (TFs) integrate into the FT-FD complex to control floral initiation in Arabidopsis (Arabidopsis thaliana). Class II CINCINNATA (CIN) TCP TFs function as transcriptional activators by directly binding to the promoters of downstream floral meristem identity genes, such as APETALA1 (AP1). In addition, these TCPs directly interact with FD, a basic Leu zipper TF that plays a critical role in photoperiodic flowering, which further activates AP1 expression. Genetic analyses indicated that class II CIN TCP TFs function synergistically with FT and FD, to positively regulate flowering in an AP1-dependent manner. Thus, our results provide compelling evidence that class II CIN TCP TFs act directly at the AP1 promoter to enhance its transcription, thus further elucidating the molecular mechanisms underlying the regulation of photoperiodic flowering in Arabidopsis.