Conjugational delivery of chromosomal integrative constructs for gene expression in the carbendazim-degrading Rhodococcus erythropolis D-1
Conjugational delivery of chromosomal integrative constructs for gene expression in the carbendazim-degrading Rhodococcus erythropolis D-1
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DOI：
10.1007/s13213-018-1382-7
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Hui Jiang
中科院分区：
文献类型：
--
作者：
Yang Tao;Wen-Guang Wang;Sheng-Hui Lu;Xin Xie;Ugit Lazzat;Naling Bai;Yuhua Zhao;Hui Jiang
Rhodococcus strains not only have been widely used in industries but also have a potential ability of producing new structural.natural products. Integration of heterologous genes into chromosomes of Rhodococcus strains for gene expression can facilitate.the studies and applications of these strains. A conjugation system was optimized in order to transfer enhanced green fluorescent.protein (EGFP) encoding gene as a reporter from Escherichia coli into Rhodococcus erythropolis D-1. The influence of three.native ribosome binding sites (RBSs) and two designed RBSs on the target protein production in R. erythropolis D-1 was also.characterized. An efficient conjugation system of R. erythropolis D-1 was established to integrate EGFP gene into its chromosome..Among of five RBSs, RBS3 showed the highest translational activity in R. erythropolis D-1.
