PROTEIN SYNTHESIS IN AMPHIBIAN OOCYTES AND EARLY EMBRYOS
PROTEIN SYNTHESIS IN AMPHIBIAN OOCYTES AND EARLY EMBRYOS
复制标题
DOI：
10.1016/0012-1606(68)90034-1
复制
发表时间：
1968-01-01
影响因子：
2.7
通讯作者：
SMITH, LD
中科院分区：
文献类型：
--
作者：
ECKER, RE;SMITH, LD
已经在从完全生长的卵巢卵母细胞到垂体诱导成熟期和受精或人工激活后数小时的所有阶段的淡色林蛙卵母细胞中研究了氨基酸掺入动力学。此外，在所有这些阶段都测量了卵母细胞中氨基酸库的大小。池的大小受到不同动物的蛋的相当大的自然变化的影响。然而，在同一阶段的卵子中，氨基酸流过池的速率以及蛋白质合成的速率相对恒定，即使取自不同的动物也是如此。未受刺激的雌性卵巢卵母细胞以及垂体刺激后约 20 小时的卵母细胞中的蛋白质合成速率非常低。此时，速率迅速上升，达到每小时每个卵母细胞约 0.5 μg 的最高水平，此后保持基本恒定。受精和人工激活对蛋白质合成都没有任何可观察到的定量影响。
Kinetics of amino acid incorporation have been studied in oocytes ofRana pipiensat all stages from the full-grown ovarian oocyte, through the period of pituitary-induced maturation and several hours beyond fertilization or artificial activation. Also, amino acid pool sizes in oocytes have been measured at all these stages. Pool sizes are subject to considerable natural variation in the eggs taken from different animals. However, the rate of flow of amino acid through the pool and, thus, the rate of protein synthesis, are relatively constant in eggs at the same stage, even when taken from different animals.The rate of protein synthesis is very low in the ovarian oocyte from an unstimulated female and in oocytes up to about 20 hours after pituitary stimulation. At this time the rate rises rapidly, reaches a maximum level of about 0.5 μg per oocyte per hour and remains essentially constant thereafter. Neither fertilization nor artificial activation have any observable quantitative effect on protein synthesis.