Identification of 18 new transcribed retrotransposons in Schistosoma mansoni
Identification of 18 new transcribed retrotransposons in Schistosoma mansoni
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DOI：
10.1016/j.bbrc.2005.05.080
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发表时间：
2005-07-22
影响因子：
3.1
通讯作者：
Verjovski-Almeida, S
中科院分区：
文献类型：
--
作者：
DeMarco, R;Machado, AA;Verjovski-Almeida, S
本文描述了18个新转录的曼氏血吸虫反转录转座子。其中9个为LTR， 8个为非LTR， 1个为Penelope-like element （PLE）反转录转座子。序列是通过mansoni est的计算机重建和cDNA末端的快速扩增获得的转录本生成的，在某些情况下，通过PCR扩增的基因组克隆测序进行补充。本文首次在曼氏血吸虫中发现了一个来自古代R2/R4/CRE转座子群的新元件。此外，一个非LTR逆转录转座子家族显示出长（40-450 bp）的3'-UTR，在这些拷贝中至少有6个不同的转录序列，5个LTR逆转录转座子具有大量转录不完整的拷贝，缺乏编码逆转录酶结构域的序列片段，4个非LTR逆转录转座子编码dna结合PHD结构域，这可能使它们具有不同的靶向性。这些结果允许对这种复杂的人类寄生虫的转录反转录转座子多样性进行全面的描述。(c) 2005爱思唯尔公司版权所有。
This work describes 18 new transcribed retrotransposons of the blood fluke Schistosoma mansoni. Among them, 9 were LTR, 8 non-LTR, and 1 Penelope-like element (PLE) retrotransposon. Sequences were generated by in silico reconstruction using S. mansoni ESTs and transcripts obtained by rapid amplification of cDNA ends, complemented in some cases by sequencing of genomic clones amplified by PCR. A novel element from the ancient R2/R4/CRE transposon group is described for the first time in S. mansoni. In addition, one non-LTR retrotransposon family displays long (40-450 bp) 3'-UTR with at least six different transcribed sequences among the copies, five LTR retrotransposons have abundantly transcribed incomplete copies lacking the sequence segment coding for the reverse transcriptase domain, and four non-LTR retrotransposons code for DNA-binding PHD domains that may give them a differential targeting. These results allow for a comprehensive description of the transcribed retrotransposon diversity of this complex human parasite. (c) 2005 Elsevier Inc. All rights reserved.