HOW MANY PHOTONS ARE NECESSARY FOR FLUORESCENCE-LIFETIME MEASUREMENTS
HOW MANY PHOTONS ARE NECESSARY FOR FLUORESCENCE-LIFETIME MEASUREMENTS
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DOI：
10.1016/0009-2614(92)87068-z
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发表时间：
1992-11-27
影响因子：
2.8
通讯作者：
WOLFRUM, J
中科院分区：
文献类型：
--
作者：
KOLLNER, M;WOLFRUM, J
在计划时间相关的单光子计数实验的荧光寿命测量与分钟的样品，最终的灵敏度的问题出现。为了回答这个问题，我们将找出需要多少探测到的光子才能在寿命估计中达到一定的精度。使用最小二乘或最大似然程序和多项式分布，寿命估计的准确性可以明确地表示为信号强度的函数。这使得人们可以确定所需的检测到的光子数量。
On planning time-correlated single photon counting experiments for fluorescence-lifetime measurements with minute samples, the question of ultimate sensitivity arises. To answer this question, we will find out how many detected photons one needs to achieve a certain accuracy in the lifetime estimate. Using-least-squares or maximum-likelihood procedures and the multinomial distribution, the accuracy of the lifetime estimation can be expressed explicitly as a function of the signal strength. This allows one to determine the required number of detected photons.