Antidiabetic and antioxidant effects of extracts from Potentilla discolor Bunge on diabetic rats induced by high fat diet and streptozotocin
Antidiabetic and antioxidant effects of extracts from Potentilla discolor Bunge on diabetic rats induced by high fat diet and streptozotocin
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DOI：
10.1016/j.jep.2010.08.053
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发表时间：
2010-11-11
影响因子：
5.4
通讯作者：
Lai, Mao-xiang
中科院分区：
文献类型：
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作者：
Zhang, Li;Yang, Jie;Lai, Mao-xiang
研究目的：翻白草（Potentilla discolor Bunge）常见于北温带和寒带地区，在中国长期用于治疗糖尿病。黄酮类化合物和三萜化合物是黄芪中的两种主要化合物。本实验旨在探讨黄芪总黄酮提取物（TFE）和总三萜提取物（TTE）对高脂饮食和链脲佐菌素诱导的糖尿病大鼠血糖、血脂及抗氧化指标的影响。材料与方法：采用高脂饮食喂养的糖尿病大鼠和链脲佐菌素诱导的糖尿病大鼠分别给予TFE和TTE治疗15 d。测试一系列参数，包括空腹血糖（FBG）、血清胰岛素（SI）、血脂、丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、糖基化血清蛋白（GSP）和一氧化氮（NO）。结果：与对照组相比，TFE或TTE治疗糖尿病大鼠的FBG和GSP浓度均降低。同时，TFE和TTE治疗的糖尿病大鼠血清总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-c）水平均低于对照组，高密度脂蛋白胆固醇（HDL-c）水平高于对照组。此外，提取物降低了糖尿病大鼠的MDA和NO水平，升高了SOD和GSH水平。组织病理学检查也表明，提取物对糖尿病大鼠β细胞有保护作用，这与SI的增加有关。结论：这两种提取物对糖尿病及其并发症的降血糖和降血脂作用可能是通过其较强的抗氧化活性和对β细胞的保护作用来实现的。2010爱思唯尔爱尔兰有限公司版权所有。
Aim of the study: Potentilla discolor Bunge, commonly found at the north temperate and boreal zone, has been used for diabetes in China for a long time. Flavonoids and triterpenoids are two major types of compounds in P. discolor. This study was designed primarily to investigate the effects of total flavonoids extract (TFE) and total triterpenoids extract (TTE) of P. discolor Bunge on blood glucose, lipid profiles and antioxidant parameters on diabetic rats induced by high fat diet and streptozotocin.Materials and methods: High fat diet-fed and streptozotocin-induced diabetic rats were treated with the TFE and TTE for 15 days, respectively. A range of parameters were tested including fasting blood glucose (FBG), serum insulin (SI), blood lipid profile, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), glycosylated serum protein (GSP), and nitric oxide (NO).Results: Diabetic rats treated with TFE or TTE had decreased concentration of FBG and GSP compared with the control group. Meanwhile, the levels of serum total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-c) in the TFE or TTE treated diabetic rats were lower, and the high-density lipoprotein cholesterol (HDL-c) level was higher than in the control diabetic rats. Furthermore, the extracts treatment decreased the MDA and NO level, while increased SOD and GSH levels in diabetic rats. Histopathologic examination also showed that the extracts have protective effects on beta-cells in diabetic rats which are supported by the increase of SI.Conclusions: All these experimental results highlighted the hypoglycemic and hypolipidemic properties of the two extracts from Potentilla discolor Bunge on diabetes and its complications, possibly through a strong antioxidant activity and a protective action on beta-cells. (C) 2010 Elsevier Ireland Ltd. All rights reserved.