Natural recombination event within the capsid genomic region leading to a chimeric strain of human enterovirus B
Natural recombination event within the capsid genomic region leading to a chimeric strain of human enterovirus B
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DOI：
10.1128/jvi.00180-07
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发表时间：
2007-09-01
影响因子：
5.4
通讯作者：
Pillet, Sylvie
中科院分区：
文献类型：
--
作者：
Bouslama, Lamjed;Nasri, Dorsaf;Pillet, Sylvie
两个菌株之间的重组是肠道病毒在单个细胞内复制的已知现象。我们描述了一株从人类粪便中回收的重组株，在对VP1和VP2编码区进行部分测序后，分别命名为柯萨奇病毒B4(CV-B4)和CV-B3。该菌株可被CV-B3特异性多克隆抗血清中和，但不能被CV-B4特异性抗血清中和。对整个结构基因组区域的核苷酸序列分析表明，在VP3衣壳基因的第1950位发生了重组事件，该重组事件发生在编码先前为CV-B3描述的2b抗原位点的区域。这一观察首次证明了两个非脊髓灰质炎病毒肠道病毒株之间在VP2-VP3-VP1衣壳区域内发生了血清型间重组。中和模式表明，主要的抗原点位于VP2蛋白内。
Recombination between two strains is a known phenomenon for enteroviruses replicating within a single cell. We describe a recombinant strain recovered from human stools, typed as coxsackievirus B4 (CV-B4) and CV-B3 after partial sequencing of the VP1 and VP2 coding regions, respectively. The strain was neutralized by a polyclonal CV-B3-specific antiserum but not by a CV-B4-specific antiserum. The nucleotide sequence analysis of the whole structural genomic region showed the occurrence of a recombination event at position 1950 within the VP3 capsid gene, in a region coding for the 2b antigenic site previously described for CV-B3. This observation evidences for the first time the occurrence of an interserotypic recombination within the VP2-VP3-VP1 capsid region between two nonpoliovirus enterovirus strains. The neutralization pattern suggests that the major antigenic site is located within the VP2 protein.