QUANTITATIVE CELL COMPOSITION OF HUMAN AND BOVINE CORPORA-LUTEA FROM VARIOUS REPRODUCTIVE STATES
QUANTITATIVE CELL COMPOSITION OF HUMAN AND BOVINE CORPORA-LUTEA FROM VARIOUS REPRODUCTIVE STATES
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DOI：
10.1095/biolreprod44.6.1148
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发表时间：
1991-06-01
影响因子：
3.6
通讯作者：
RAO, CV
中科院分区：
文献类型：
--
作者：
LEI, ZM;CHEGINI, N;RAO, CV
采用计算机化的基于视频的交互式Bioquant图像分析系统IV和光学显微镜免疫细胞化学研究了不同生殖状态的人和牛黄体（CL）的细胞组成。人和牛CL含有更多的非黄体细胞比黄体细胞。与牛CL相比，人CL含有较少数量的黄体细胞和较多数量的非黄体细胞。无论生殖状态如何，人CL包含的小黄体细胞多于大黄体细胞。在所有的生殖状态下，除了在黄体晚期，牛CL也含有更多的小黄体细胞比大黄体细胞。人CL中所有细胞的平均大小小于牛CL。在黄体中期和晚期，人CL比牛CL含有更多的血管空间，从黄体早期到中期，黄体细胞数量增加，非黄体细胞数量减少，到黄体晚期，黄体细胞数量减少，非黄体细胞数量增加。在人黄体中，小黄体细胞和大黄体细胞的数量变化首先发生在黄体早期到中期，而在牛黄体中，这种变化直到黄体晚期才发生。人类大黄体细胞和牛小黄体细胞的平均大小没有变化，而其他细胞的大小在人类和牛CL的不同生殖状态下发生变化。妊娠晚期人CL的细胞组成与黄体中晚期相似，而妊娠早期牛CL的细胞组成与黄体中期相似，人CL中巨噬细胞的数量从黄体早期到中期和从黄体中期到晚期逐渐增多。另一方面，成纤维细胞的数量从黄体早期到中期减少，然后在黄体晚期CL几乎增加一倍。血管空间增加，从早期到中期的黄体期，其次是一个相当大的下降，在黄体晚期的人和牛CL。
The cell composition of human and bovine corpora lutea (CL) from various reproductive states was investigated by computerized video-based interactive Bioquant image analysis system IV and by light microscope immunocytochemistry. Human and bovine CL contained more nonluteal cells than luteal cells. Human CL contained a lower number of luteal and a greater number of nonluteal cells than bovine CL. Regardless of the reproductive state, human CL contained more small luteal cells than large luteal cells. In all reproductive states except in the late luteal phase, the bovine CL also contained more small luteal cells than large luteal cells. The average sizes of all the cells in human CL were smaller than in bovine CL. Human CL contained more vascular space than bovine CL during mid and late luteal phases.The number of luteal cells increased and nonluteal cells decreased from early to mid luteal phase, and then luteal cells decreased and nonluteal cells increased in late luteal phase and in degenerating human and bovine CL. While the change of number of small and large luteal cells first occurred from early to mid luteal phase in human CL, it did not take place until the late luteal phase in bovine CL. The average size of large luteal cells in humans and of small luteal cells in cattle did not change, whereas size of the other cells changed in different reproductive states in both human and bovine CL. The cell composition of term pregnancy human CL was similar to mid or late luteal phase, whereas the cell composition of early pregnancy bovine CL was similar to mid luteal phase.The number of macrophages in human CL increased from early to mid luteal phase and from mid to late luteal phase. The number of fibroblasts, on the other hand, decreased from early to mid luteal phase and then nearly doubled in late luteal phase CL. The vascular space increased from early to mid luteal phase, followed by a considerable decline in late luteal phase human and bovine CL.