A high-speed atomic force microscope for studying biological macromolecules
A high-speed atomic force microscope for studying biological macromolecules
复制标题
DOI：
10.1073/pnas.211400898
复制
发表时间：
2001-10-23
影响因子：
11.1
通讯作者：
Toda, A
中科院分区：
文献类型：
--
作者：
Ando, T;Kodera, N;Toda, A
原子力显微镜（AFM）是一种对附着在基底上并置于水溶液中的单个生物分子进行成像的强大工具。然而，目前它受限于连续记录高分辨率图像的速度。我们试图显著提高原子力显微镜的扫描速度，以便将来它可用于研究生物分子的动态行为。为此，我们开发了一种高速扫描器（在高达60 kHz的频率下无共振振动）、具有高共振频率（450 - 650 kHz）和小弹簧常数（150 - 280 pN/nm）的小悬臂、一种物镜式偏转检测装置以及几种宽带宽的电子设备。将这些不同的设备集成在一起，制造出了一种原子力显微镜，它能够在80毫秒内获取一幅100×100像素²的图像，因此能够生成一部由水溶液中样品的许多连续图像（间隔80毫秒）组成的影片。这通过对在云母上移动的肌球蛋白V分子进行成像得到了证明（见http://www.s.kanazawa - u.ac.jp/phys/biophys/bmv - movie.htm）。
The atomic force microscope (AFM) is a powerful tool for imaging individual biological molecules attached to a substrate and placed in aqueous solution. At present, however, it is limited by the speed at which it can successively record highly resolved images. We sought to increase markedly the scan speed of the AFM, so that in the future it can be used to study the dynamic behavior of biomolecules. For this purpose, we have developed a high-speed scanner, free of resonant vibrations up to 60 kHz, small cantilevers with high resonance frequencies (450-650 kHz) and small spring constants (150-280 pN/nm), an objective-lens type of deflection detection device, and several electronic devices of wide bandwidth. Integration of these various devices has produced an AFM that can capture a 100 X 100 pixel(2) image within 80 ms and therefore can generate a movie consisting of many successive images (80-ms intervals) of a sample in aqueous solution. This is demonstrated by imaging myosin V molecules moving on mica (see http://www.s.kanazawa-u.ac.jp/phys/biophys/bmv-movie.htm).