Mutation affecting peptide bond formation in nikkomycin biosynthesis.
Mutation affecting peptide bond formation in nikkomycin biosynthesis.
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DOI：
10.1016/s0006-291x(88)80898-2
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发表时间：
1988-10
影响因子：
3.1
通讯作者：
P. Engel;A. Ullah
中科院分区：
文献类型：
--
作者：
P. Engel;A. Ullah
尼克霉素是一种udp - n -乙酰氨基葡萄糖的核苷肽类似物，是一种有效的几丁质合成酶抑制剂，由腱链霉菌产生。用高效液相色谱法（HPLC）比较了非生产突变体nikkomycin培养液中发酵产物的含量。尼克霉素C和D分别是尼克霉素Z的糖元和糖元。这表明突变影响了nikkomycin C和D之间形成肽键的能力，这是合成具有生物活性的nikkomycin z的最终步骤。这也是第一个记录突变影响nikkomycin生物合成特定步骤的案例。
Nikkomycin, a nucleoside-peptide analog of UDP-N-acetylglucosamine, is a potent chitin synthase inhibitor produced by the bacteriumStreptomyces tendae. The HPLC profile of fermentation products in culture broths of a non-producing mutant, Nik 15, was compared with nikkomycin standards. Nikkomycin C and D, the glycone and aglycone moieties, respectively, of nikkomycin Z accumulated. This indicates the mutation affects the capacity to form a peptide bond between nikkomycin C and D, which is here proposed to be the terminal step in the synthesis of the biologically active nikkomycin Z. This is also the first documented case of a mutation affecting a specific step in nikkomycin biosynthesis.