The value of CEP55 gene as a diagnostic biomarker and independent prognostic factor in LUAD and LUSC
The value of CEP55 gene as a diagnostic biomarker and independent prognostic factor in LUAD and LUSC
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DOI：
10.1371/journal.pone.0233283
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发表时间：
2020-05-21
期刊：
影响因子：
3.7
通讯作者：
Yu, Guangmao
中科院分区：
文献类型：
--
作者：
Fu, Linhai;Wang, Haiyong;Yu, Guangmao
目的探讨CEP55作为肺腺癌（LUAD）和肺鳞癌（LUSC）诊断标志物和独立预后因子的价值，并分析其共表达基因和相关信号通路。利用cBioPortal挖掘LUAD和LUSC中CEP55的共表达基因，并利用KEGG和GO进行富集。建立受试者工作特征（ROC）曲线，评价CEP55作为诊断和预后因子的价值。采用卡方检验评价CEP55表达与临床病理特征之间的关系。构建诊断和预后检测的ROC曲线。单因素和多因素考克斯回归模型分析预后值。结果与正常肺组织相比，CEP 55表达在LUAD和LUSC中均显著上调。ROC曲线分析显示CEP55可作为LUAD（AUC = 0.969）和LUSC（AUC = 0.994）的有效诊断指标。当选择CEP55基因作为独立的预后因素时，CEP55高表达对LUAD患者的OS和RFS更不利（P0.05）。结论CEP55可作为LUAD和LUSC的诊断标志物，但仅作为LUAD的独立预后因子，而不是LUSC。
ObjectiveTo investigate the value of CEP55 as a diagnostic marker and independent prognostic factor in lung adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC), and to analyze its co-expression genes and related signaling pathways.MethodsTCGA database and GEO database were used to analyze the expression of CEP55 in LUAD and LUSC compared with normal tissues. The co-expression genes of CEP55 in LUAD and LUSC were excavated by cBioPortal and enriched by KEGG and GO. Establishing Receiver operating characteristic (ROC) curve to evaluate the value of CEP55 as a diagnostic and prognostic factor. The association between CEP55 expression and the clinicopathological features was evaluated using chi(2) tests. ROC curves for diagnosis and prognosis detection were constructed. Prognostic values were analyzed by univariate and multivariate Cox regression models.ResultsCompared with normal lung tissues, CEP55 expression was significantly upregulated in both LUAD and LUSC. ROC curve analysis showed that CEP55 could be used as an effective diagnostic target for LUAD (AUC = 0.969) and LUSC (AUC = 0.994). When CEP55 gene was selected as an independent prognostic factor, high expression of CEP55 was more disadvantageous to OS and RFS of LUAD patients (P0.05). The number of co-expression genes of CEP55 in LUAD is more than that in LUSC, and is related to cell cycle, DNA replication and P53 signaling pathway.ConclusionCEP55 can be used as a diagnostic marker for LUAD and LUSC, but only as an independent prognostic factor for LUAD rather than LUSC.