Network-mediated responses of ON ganglion cells to electric stimulation become less consistent across trials during retinal degeneration.
Network-mediated responses of ON ganglion cells to electric stimulation become less consistent across trials during retinal degeneration.
复制标题
在视网膜变性期间，ON神经节细胞对电刺激的网络介导反应在各个试验中变得不太一致。
DOI：
10.1109/embc.2017.8037271
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
MaesoonIm
中科院分区：
文献类型：
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作者：
Jae-IkLee;Fried,ShelleyI;MaesoonIm
正在开发微电子视网膜假体，以恢复因视网膜色素变性和年龄相关性黄斑变性等外部视网膜变性疾病而失明的个人的视力。不幸的是，这些设备恢复的视力质量仍然有限。为了提高视觉诱发的质量，我们研究了视网膜神经元对电刺激的反应。我们以前的工作表明，通过视网膜网络介导的反应是可重复的，具有高的时间精度，即使是在刺激开始后>100 ms发生的尖峰。因为它们是通过网络产生的，所以重要的是要了解这种可靠性是否在退化的视网膜中发生变化。在这里，我们研究了在几个不同阶段的退化反应的变异性：出生后第14天（P14），P18，P31和P60在一个完善的退化小鼠模型（rd10）。多次记录ON α RGC对相同电刺激的尖峰响应。我们发现，在视网膜变性的过程中，试验间的变异性增加。这一发现可能有助于解释使用这些设备的受试者的诱发视觉质量的变化。
Microelectronic retinal prostheses are being developed to restore sight in individuals blinded by outer retinal degenerative diseases such as retinitis pigmentosa and age-related macular degeneration. Unfortunately, the quality of vision restored by these devices is still limited. To improve the quality of elicited vision, our group studies the responses of retinal neurons to electric stimulation. Our previous work showed that responses mediated through the retinal network are reproducible with high temporal precision, even for spikes that occur >100 ms after stimulus onset. Because they arise through the network, it is important to understand whether such reliability changes in the degenerate retina. Here, we examined response variability at several different stages of degeneration: postnatal day 14 (P14), P18, P31 and P60 in a well-established mouse model of degeneration (rd10). Spiking responses of ON alpha RGCs were recorded multiple times to an identical electric stimulus. We found that the trial-to-trial variability increased over the course of retinal degeneration. This finding may help to explain the reported variability in the quality of elicited vision across subjects using these devices.