Statistical analysis of vesicle morphology dynamics based on free energy landscape
Statistical analysis of vesicle morphology dynamics based on free energy landscape
复制标题
基于自由能景观的囊泡形态动力学统计分析
作者：
S. Tsuda;H. Suzuki;T. Yomo
在这里，我们提出了一种方法来重建有效的自由能景观（FELs）的脂质囊泡从大量的显微镜图像的统计分析。这种方法，不仅允许我们定义可能的能量景观，而且还突出了少数囊泡形状，否则隐藏在大多数。当与变形脂质囊泡的时间演化比较时，发现变形囊泡的轨迹与重建的景观一致，其中少数形状起着关键作用。当与理论模型相比，它揭示了在重建的自由电子激光特征的囊泡形状与理论预测的形状一致。这些结果表明，自由电子激光分析可以是一个有用的工具，调查的形态动力学的脂质囊泡，结合其他分析方法。
We here present a method to reconstruct effective free energy landscapes (FELs) of lipid vesicles from the statistical analysis of a large number of microscope images. This method, not only allows us to define possible energy landscapes, but also highlights minority vesicle shapes that were otherwise hidden in the majority. When compared with temporal evolution of deforming lipid vesicles, it was found that the trajectory of deforming vesicles was in accordance with the reconstructed landscape, in which the minority shapes play a key role. When compared with theoretical models, it revealed that the vesicle shapes characterised in the reconstructed FELs were consistent with the theoretically predicted shapes. These results suggest that the FEL analysis can be a useful tool to investigate the morphological dynamics of lipid vesicles, in conjunction with other analytical methods.