Improvement of a rapid diagnosis kit to detect either influenza a or B virus infections
Improvement of a rapid diagnosis kit to detect either influenza a or B virus infections
复制标题
DOI：
10.1292/jvms.68.35
复制
发表时间：
2006-01-01
影响因子：
1.2
通讯作者：
Kida, H
中科院分区：
文献类型：
--
作者：
Bai, GR;Sakoda, Y;Kida, H
为提高检测甲型或乙型流感病毒特异性核蛋白（NP）快速诊断试剂盒ESPLINE (R) INFLUENZA A&B的灵敏度，利用新建立的针对各自NP分子的单克隆抗体（mab）开发了ESPLINE INFLUENZA A&B- n试剂盒。单克隆抗体FVA2-11和FrB1-03分别识别甲型流感病毒NP分子的59-130aa氨基酸区和乙型流感病毒NP分子的72-191aa氨基酸区。新试剂盒检测甲型和乙型流感病毒抗原的检出限为10(2.0)~ 10(2.7)pfu/次，比原试剂盒提高了4-1000倍。重要的是，该试剂盒检测了已知血凝素（HA）亚型（H1-H15）的每一种甲型流感病毒，包括最近从亚洲人源和禽源分离的H5N1病毒。该试剂盒还检测了所有15种代表性的乙型流感病毒株。因此，ESPLINE (R)流感a和B- n是一种快速、高度敏感和特异性强的试剂盒，可用于诊断甲型或乙型流感病毒感染。
To improve the sensitivity of a kit, ESPLINE (R) INFLUENZA A&B for rapid diagnosis of influenza by detecting influenza A or B virus specific nucleoproteins (NP), the ESPLINE (R) INFLUENZA A&B-N was developed by using newly established monoclonal antibodies (MAbs) to the respective NP molecule. MAbs FVA2-11 and FrB1-03 recognize the epitope on the amino acid region 59-130aa of the NP molecule of influenza A virus, and that on the region 72-191aa of the NP of influenza B virus, respectively. The new kit detected influenza A and B virus antigens with a detection limit of 10(2.0)-10(2.7) pfu/test, which is 4-1000 times higher than that of the original kit. Importantly, this kit detected each of influenza A viruses of the known hemagglutinin (HA) subtypes (H1-H15) including the H5N1 viruses recently isolated from human and avian sources in Asia. The kit also detected all of the 15 representative influenza B virus strains tested. The ESPLINE (R) INFLUENZA A&B-N is thus a rapid and highly sensitive and specific kit for the diagnosis of either influenza A or B virus infections.