Major alterations in transcript profiles between C3-C4 and C4 photosynthesis of an amphibious species Eleocharis baldwinii
Major alterations in transcript profiles between C3-C4 and C4 photosynthesis of an amphibious species Eleocharis baldwinii
复制标题
两栖物种荸荠 C3→C4 和 C4 光合作用转录谱的主要变化
DOI：
10.1007/s11103-014-0215-8
复制
发表时间：
2014-09-01
影响因子：
5.1
通讯作者：
Lin, Yongjun
中科院分区：
文献类型：
--
作者：
Chen, Taiyu;Zhu, Xin-Guang;Lin, Yongjun
将C4光合代谢工程化到C3作物中被认为是提高作物生产力的主要策略，并且澄清C4光合作用的进化过程可以帮助更好地使用该策略。在此，Eleocharis baldwinii，一种在环境或阿坝处理下可以从C3-C4状态诱导C4光合作用的物种，被用于鉴定C3-C4到C4过程中的主要转录修饰。转录组学比较表明，除了C4核心途径的主要差异外，糖酵解、柠檬酸代谢和蛋白质合成途径在C4光合状态诱导过程中也发生了显著变化。在C4光合状态下，许多转运蛋白的转录本显着增加，不仅包括代谢物转运蛋白，还包括离子转运蛋白。C3-C4和C4光合状态下，许多功能未知的候选调控基因差异表达。阿坝、生长素信号和DNA甲基化在C4光合作用调控中起着重要作用。综上所述，通过研究同一物种的不同光合状态，本工作提供了C3-C4和C4光合作用之间的主要转录差异，其中许多转录差异可能与C4发育有关，因此是反向遗传学研究的潜在目标。
Engineering C4 photosynthetic metabolism into C3 crops is regarded as a major strategy to increase crop productivity, and clarification of the evolutionary processes of C4 photosynthesis can help the better use of this strategy. Here, Eleocharis baldwinii, a species in which C4 photosynthesis can be induced from a C3–C4 state under either environmental or ABA treatments, was used to identify the major transcriptional modifications during the process from C3–C4 to C4. The transcriptomic comparison suggested that in addition to the major differences in C4 core pathway, the pathways of glycolysis, citrate acid metabolism and protein synthesis were dramatically modified during the inducement of C4 photosynthetic states. Transcripts of many transporters, including not only metabolite transporters but also ion transporters, were dramatically increased in C4 photosynthetic state. Many candidate regulatory genes with unidentified functions were differentially expressed in C3–C4 and C4 photosynthetic states. Finally, it was indicated that ABA, auxin signaling and DNA methylation play critical roles in the regulation of C4 photosynthesis. In summary, by studying the different photosynthetic states of the same species, this work provides the major transcriptional differences between C3–C4 and C4 photosynthesis, and many of the transcriptional differences are potentially related to C4 development and therefore are the potential targets for reverse genetics studies.