Tyrosine phosphorylation of membrane-associated tubulin in nerve growth cones enriched in pp60c-src.
Tyrosine phosphorylation of membrane-associated tubulin in nerve growth cones enriched in pp60c-src.
复制标题
富含 pp60c-src 的神经生长锥中膜相关微管蛋白的酪氨酸磷酸化。
DOI：
10.1002/9780470513927.ch5
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Matten,WT
中科院分区：
文献类型：
--
作者：
Maness,PF;Matten,WT
c - srproto -癌基因（pp60c - src）的产物是酪氨酸特异性蛋白激酶，在神经发育的两个阶段表达。在有丝分裂后处于终末分化的神经细胞中，pp60c - sri在神经生长锥和近端轴突轴的膜中含量较高。膜相关形式的α -和β -微管蛋白是体内生长锥膜中主要的磷酸酪氨酸修饰蛋白。Pp60c‐在体外磷酸化纯化的、未组装的微管蛋白亚单位，抑制其聚合成微管的能力。可以想象，生长锥中pp60c‐srin的微管蛋白磷酸化可能通过改变细胞对基质的粘附来调节神经突的延伸。
The product of the c‐srcproto‐oncogene (pp60c‐src) is a tyrosine‐specific protein kinase that is expressed in two phases of neural development. In post‐mitotic neuronal cells undergoing terminal differentiation, pp60c‐srcis present at high levels in the membrane of nerve growth cones and proximal axon shafts. Membrane‐associated forms of α‐ and β‐tubulin are the major phosphotyrosine‐modified proteins in growth cone membranesin vivo. pp60c‐srcphosphorylates purified, unassembled tubulin subunitsin vitro, inhibiting their ability to polymerize into microtubules. It is conceivable that tubulin phosphorylation by pp60c‐srcin the growth cone may regulate neurite extension by altering adhesion of cells to the substratum.