Hormonal regulation of stability of glutamine synthetase mRNA in cultured 3T3-L1 adipocytes.
Hormonal regulation of stability of glutamine synthetase mRNA in cultured 3T3-L1 adipocytes.
复制标题
培养的 3T3-L1 脂肪细胞中谷氨酰胺合成酶 mRNA 稳定性的激素调节。
DOI：
10.1042/bj2670241
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Bhandari,B
中科院分区：
文献类型：
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作者：
Saini,K;Thomas,P;Bhandari,B
在3 T3-L1脂肪细胞中，谷氨酰胺合成酶（GS; EC 6.3.1.2）受到地塞米松、胰岛素和二丁酰环AMP（Bt 2cAMP）的调节。地塞米松增加GS-mRNA含量和GS基因转录，而胰岛素和Bt 2cAMP阻止这些增加。研究了这些调节剂对GS-mRNA稳定性控制的影响。我们在这里报告，GS mRNA的半衰期约为110分钟。Bt 2cAMP增加GS-mRNA降解大于2倍（半衰期50分钟），而胰岛素或地塞米松对GS-mRNA的稳定性几乎没有影响。Bt 2cAMP对GS基因表达的下调涉及基因转录水平和mRNA稳定性水平的协调反应。然而，胰岛素和地塞米松调节GS基因在培养的脂肪细胞中表达的分子机制仍有待阐明。
In 3T3-L1 adipocytes, glutamine synthetase (GS; EC 6.3.1.2) is subject to regulation by dexamethasone, insulin and dibutyryl cyclic AMP (Bt2cAMP). Dexamethasone increases GS-mRNA content and GS-gene transcription, whereas insulin and Bt2cAMP prevent these increases. The effects of these modulators on the control of GS-mRNA stability were investigated. We report here that GS mRNA has a half-life of about 110 min. Bt2cAMP increases GS-mRNA degradation by greater than 2-fold (half-life 50 min), whereas insulin or dexamethasone have little effect on GS-mRNA stability. Down-regulation of GS-gene expression by Bt2cAMP will involve a co-ordinate response at the level of gene transcription and mRNA stability. However, the molecular mechanisms by which insulin and dexamethasone regulate GS-gene expression in cultured adipocytes remains to be elucidated.