Voiding pattern analysis as a surrogate for cystometric evaluation in uroplakin II knockout mice
Voiding pattern analysis as a surrogate for cystometric evaluation in uroplakin II knockout mice
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DOI：
10.1016/j.juro.2007.12.039
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发表时间：
2008-05-01
影响因子：
6.6
通讯作者：
Christ, George J.
中科院分区：
文献类型：
--
作者：
Hodges, Steve J.;Zhou, Ge;Christ, George J.
目的：之前的研究表明，uroplakin II 的缺失会导致尿路功能障碍，包括膀胱输尿管反流和肾脏异常，以及排尿模式改变。我们开发了一种简单的膀胱功能替代测量方法，使用 uroplakin 11 敲除小鼠动物模型中尿液排尿模式的紫外线可视化。 材料和方法：通过膀胱测压分析和排尿模式标记对三只雄性和 3 只雌性 WT 小鼠以及 3 只雄性和 3 只雌性 uroplakin II 敲除小鼠进行评估。排尿模式标记由独立观察者根据排尿尿液的分散程度按 1 至 5 的等级进行分级。然后使用统计分析将同一小鼠的排尿分散等级与膀胱测压参数相关联。结果：排尿模式标记的分散程度与膀胱功能的多种测量相关。具体而言，观察到的排尿模式的皮尔逊相关系数与这些小鼠在清醒膀胱测压期间进行的基线压力、阈值压力和排尿间压力测量高度相关（p < 0.05）。结论：小鼠排尿模式的紫外线可视化与标准膀胱测压评估的某些测量值密切相关。因此，该方法提供了一种简单、无创的评估小鼠膀胱功能的方法。这种方法的实施有可能实现高通量分析的自动化，可以加速针对膀胱疾病/功能障碍的某些重要方面的新疗法的开发。
Purpose: Previous study has shown that the absence of uroplakin II can cause urinary tract dysfunction, including vesicoureteral reflux and renal abnormalities, as well as micturition pattern changes. We developed a simple surrogate measure of bladder function using ultraviolet visualization of urinary voiding patterns in a uroplakin 11 knockout mouse animal model.Materials and Methods: Three male and 3 female WT mice, and 3 male and 3 female uroplakin II knockout mice were evaluated by cystometric analysis and voiding pattern markings. Voiding pattern markings were graded by independent observers on a scale of 1 to 5 according to the degree of dispersion of voided urine. Statistical analysis was then used to correlate voiding dispersion grades with cystometric parameters in the same mice.Results: The degree of dispersion of voiding pattern markings correlated with several measures of bladder function. Specifically the Pearson correlation coefficients for the observed voiding patterns highly correlated with baseline pressure, threshold pressure and intermicturition pressure measurements made during conscious cystometry in these mice (p < 0.05).Conclusions: Ultraviolet visualization of urinary voiding patterns of mice correlated well with certain measures of standard cystometric evaluations. As such, this method provides a simple, noninvasive method of evaluating mouse bladder function. Implementation of this methodology, which can potentially be automated for high throughput analysis, can accelerate the development of novel therapy for certain important aspects of bladder disease/dysfunction.