The Chemical Sensitivity and Electrical Activity of Individual Olfactory Sensory Neurons to a Range of Sex Pheromones and Food Odors in the Goldfish
The Chemical Sensitivity and Electrical Activity of Individual Olfactory Sensory Neurons to a Range of Sex Pheromones and Food Odors in the Goldfish
复制标题
金鱼个体嗅觉感觉神经元对一系列性信息素和食物气味的化学敏感性和电活动
DOI：
10.1093/chemse/bjy016
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Sorensen Peter W
中科院分区：
文献类型：
--
作者：
Sato Koji;Sorensen Peter W
虽然已经确定硬骨鱼的嗅觉上皮使用5种类型的嗅觉感觉神经元（OSNs）检测至少6类生物学相关的气味，但是关于单个OSNs的特异性以及它们如何编码天然气味的身份的了解很少。在这项研究中，我们使用在体细胞外单单位记录检查的气味反应和生理特征的109个单独的OSN在成熟的雄性金鱼的广泛的生物气味，包括喂养刺激（氨基酸，多胺，核苷酸），性信息素（性类固醇，前列腺素[PG]），和一个假定的社会线索（胆汁酸）。61个OSN是化学敏感的，其中超过一半（36个）对氨基酸，7个对多胺，7个对核苷酸，5个对胆汁酸，9个对PG，7个对性类固醇有反应。大约四分之一的氨基酸敏感单位也响应多胺或核苷酸。6个氨基酸敏感单位中有3个对1种以上的氨基酸化合物有反应，5个性信息素敏感单位只检测到1种性信息素。虽然信息素敏感的OSNs也响应腺苷酸环化酶激活剂，毛喉素，氨基酸敏感的OSNs响应毛喉素或磷脂酶C激活剂，丙咪嗪。大多数OSN对气味剂和激活剂有兴奋反应。我们的研究结果表明，信息素信息编码的OSN专门调谐到单一的性信息素，并采用腺苷酸环化酶，暗示的标记线组织，而食物信息编码的OSN的组合，使用腺苷酸环化酶和磷脂酶C，往往不太具体调谐。
Although it is well established that the olfactory epithelium of teleost fish detects at least 6 classes of biologically relevant odorants using 5 types of olfactory sensory neurons (OSNs), little is understood about the specificity of individual OSNs and thus how they encode identity of natural odors. In this study, we usedin vivoextracellular single-unit recording to examine the odor responsiveness and physiological characteristics of 109 individual OSNs in mature male goldfish to a broad range of biological odorants including feeding stimuli (amino acids, polyamines, nucleotides), sex pheromones (sex steroids, prostaglandins [PGs]), and a putative social cue (bile acids). Sixty-one OSNs were chemosensitive, with over half of these (36) responding to amino acids, 7 to polyamines, 7 to nucleotides, 5 to bile acids, 9 to PGs, and 7 to sex steroids. Approximately a quarter of the amino acid-sensitive units also responded to polyamines or nucleotides. Three of 6 amino acid-sensitive units responded to more than 1 amino acid compound, and 5 sex pheromone-sensitive units detected just 1 sex pheromone. While pheromone-sensitive OSNs also responded to the adenylyl cyclase activator, forskolin, amino acid-sensitive OSNs responded to either forskolin or a phospholipase C activator, imipramine. Most OSNs responded to odorants and activators with excitation. Our results suggest that pheromone information is encoded by OSNs specifically tuned to single sex pheromones and employ adenylyl cyclase, suggestive of a labeled-line organization, while food information is encoded by a combination of OSNs that use both adenylyl cyclase and phospholipase C and are often less specifically tuned.