Quantitative expression analysis of GH3, a gene induced by plant growth regulator herbicides in soybean
Quantitative expression analysis of GH3, a gene induced by plant growth regulator herbicides in soybean
复制标题
DOI：
10.1021/jf035134l
复制
发表时间：
2004-02-11
影响因子：
6.1
通讯作者：
Riechers, DE
中科院分区：
文献类型：
--
作者：
Kelley, KB;Lambert, KN;Riechers, DE
类似于脱靶植物生长调节剂 (PGR) 除草剂损伤的症状在大豆田中很常见，但致病因子通常很难识别。使用实时 RT-PCR 对 PGR 除草剂损伤的大豆叶片中生长素调节的大豆基因 GH3 的表达进行定量。分析 GH3 的表达以确定其是否适合用于诊断测定，以确定 PGR 除草剂是否是造成损伤的原因。治疗后 3 天内 (DAT) GH3 被麦草畏高度诱导，并在 7 DAT 时保持高水平，但在 17 DAT 时诱导低得多。 GH3 在 7 DAT 时也被麦草畏 + 二氟吡吡高度诱导，其他 PGR 除草剂二氯吡啶酸和 2,4-D 也有较小程度的诱导。非 PGR 除草剂草甘膦、咪草烟和氟磺胺草醚没有显着诱导 GH3 表达高于低组成水平。这些结果表明，基于生长素响应基因的过表达的 PGR 除草剂损伤的诊断测定是可行的，并且 GH3 是可以开发诊断测定的潜在候选者。然而，GH3 表达的时程分析表明，该测定在接触除草剂后的有限时间内有效。
Symptoms resembling off-target plant growth regulator (PGR) herbicide injury are frequently found in soybean fields, but the causal agent is often difficult to identify. The expression of GH3, an auxin-regulated soybean gene, was quantified from soybean leaves injured by PGR herbicides using real-time RT-PCR. Expression of GH3 was analyzed to ascertain its suitability for use in a diagnostic assay to determine whether PGR herbicides are the cause of injury. GH3 was highly induced by dicamba within 3 days after treatment (DAT) and remained high at 7 DAT, but induction was much lower at 17 DAT. GH3 was also highly induced at 7 DAT by dicamba + diflufenzopyr, and to a lesser extent by the other PGR herbicides clopyralid and 2,4-D. The non-PGR herbicides glyphosate, imazethapyr, and fomesafen did not significantly induce GH3 expression above a low constitutive level. These results indicate that a diagnostic assay for PGR herbicide injury based on overexpression of auxin-responsive genes is feasible, and that GH3 is a potential candidate from which a diagnostic assay could be developed. However, time course analysis of GH3 expression indicates the assay would be effective for a limited time after exposure to the herbicide.