Sulphation by cultured cells. Cysteine, cysteinesulphinic acid and sulphite as sources for proteoglycan sulphate.
Sulphation by cultured cells. Cysteine, cysteinesulphinic acid and sulphite as sources for proteoglycan sulphate.
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培养细胞的硫酸化。
DOI：
10.1042/bj2520305
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Silbert,JE
中科院分区：
文献类型：
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作者：
Humphries,DE;Silbert,CK;Silbert,JE
对牛主动脉平滑肌细胞、牛主动脉内皮细胞和IMR-90人胚肺成纤维细胞进行了检测，以确定其使用半胱氨酸或半胱氨酸代谢物作为硫酸盐（SO 4）来源的能力。将细胞在含有[3 H]葡糖胺加0.2 mM-胱氨酸、0.3 mM-半胱氨酸亚磺酸或0.3 mM-亚硫酸盐（SO 3）的SO 4耗尽培养基中孵育。发现由不同细胞产生的[3 H]硫酸软骨素在这些条件下的硫酸化程度有很大差异。一种平滑肌细胞系有效地利用半胱氨酸作为SO 4来源，从而产生高度硫酸化的硫酸软骨素。IMR-90成纤维细胞在这些条件下产生部分硫酸化的硫酸软骨素，而另一种平滑肌细胞系不能利用半胱氨酸，但可以利用半胱氨酸亚磺酸作为部分SO 4源。与上述细胞相反，内皮细胞不能使用半胱氨酸或半胱氨酸亚磺酸作为SO 4的来源，并且几乎不含SO 4地产生软骨素。所有的电池都能够利用SO 3。在含有[35 S]半胱氨酸的SO 4耗尽培养基中孵育细胞证实，只有第一行平滑肌细胞可以将大量的[35 S]半胱氨酸转化为35 SO 4。此外，添加0.4 mM无机SO 4不会抑制这些细胞从半胱氨酸产生SO 4。
Bovine aortic smooth-muscle cells, bovine aortic endothelial cells, and IMR-90 human embryonic lung fibroblasts were tested to determine their ability to use cysteine or cysteine metabolites as a source of sulphate (SO4). Cells were incubated in SO4-depleted medium containing [3H]glucosamine plus 0.2 mM-cystine, 0.3 mM-cysteinesulphinic acid or 0.3 mM-sulphite (SO3). The [3H]chondroitin sulphate produced by the different cells was found to vary considerably in degree of sulphation under these conditions. One line of smooth-muscle cells utilized cysteine effectively as a SO4 source and thus produced chondroitin sulphate which was highly sulphated. IMR-90 fibroblasts produced partly sulphated chondroitin sulphate under these conditions, while another smooth-muscle cell line could not utilize cysteine, but could utilize cysteinesulphinic acid as a partial SO4 source. In contrast with the above cells, endothelial cells could not use cysteine or cysteinesulphinic acid as a source of SO4 and produced chondroitin with almost no SO4. All of the cells were able to utilize SO3. Incubation of the cells in the SO4-depleted medium containing [35S]cysteine confirmed that only the first line of smooth-muscle cells could convert significant amounts of [35S]cysteine to 35SO4. Furthermore, the addition of 0.4 mM inorganic SO4 did not inhibit the production of SO4 from cysteine by these cells.