THE EFFECT OF INSULIN DEFICIENCY, DIETARY-PROTEIN INTAKE, AND PLASMA AMINO-ACID CONCENTRATIONS ON BRAIN AMINO-ACID LEVELS IN RATS
THE EFFECT OF INSULIN DEFICIENCY, DIETARY-PROTEIN INTAKE, AND PLASMA AMINO-ACID CONCENTRATIONS ON BRAIN AMINO-ACID LEVELS IN RATS
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DOI：
10.1139/y85-084
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发表时间：
1985-01-01
影响因子：
2.1
通讯作者：
ANDERSON, GH
中科院分区：
文献类型：
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作者：
GLANVILLE, NT;ANDERSON, GH
本文研究了糖尿病(链脲佐菌素，65 mg/kg)、膳食蛋白质摄入量(15-60%)和血浆氨基酸浓度对大鼠脑内大分子中性氨基酸水平的影响。20d后，糖尿病大鼠血浆蛋氨酸、支链氨基酸(BCAA)、Val、Ile、Leu浓度升高。在脑组织中，Met和Val水平升高，而Thr、Tyr和Typ水平下降。蛋白质摄入量的增加促进了正常大鼠糖尿病样血浆氨基酸模式的形成，而糖尿病动物则增强了这种模式。除苏氨酸、甘氨酸、Val和Tyr外，对脑内氨基酸水平影响不大。在糖尿病动物中，计算的脑内流率与苏氨酸、蛋氨酸、酪氨酸和Try的实际脑浓度之间存在很好的相关性。脑内流率与脑内支链氨基酸水平之间无相关性。糖尿病患者的大脑氨基酸模式代表了胰岛素不足和饮食结构对血浆氨基酸水平以及大脑自身新陈代谢适应的综合影响。
The effect of diabetes (streptozotocin, 65 mg/kg i.p.), dietary protein intake (15-60%), and plasma amino acid concentrations on brain large neutral amino acid levels in rats was examined. After 20 days, the plasma concentrations of Met and the branched chain amino acids (BCAA), Val, Ile, and Leu were increased in diabetic rats. In brain tissue, Met and Val levels were increased but Thr, Tyr, and Typ concentrations were depressed. Increased protein consumption promoted a diabetic-like plasma amino acid pattern in normal rats while enhancing that of diabetic animals. With the exception of Thr, Gly, Val and Tyr, there was little effect on brain amino acid levels. A good association was found between the calculated brain influx rate and the actual brain concentration of Thr, Met, Tyr, and Try in diabetic animals. There was no correlation between brain influx rate and brain BCAA levels. The brain amino acid pattern in diabetes represents the combined effect of insulin insufficiency and composition of the diet ingested on plasma amino levels as well as metabolic adaptation within the brain itself.