Optical Phase Measurements of Disorder Strength Link Microstructure to Cell Stiffness
Optical Phase Measurements of Disorder Strength Link Microstructure to Cell Stiffness
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DOI：
10.1016/j.bpj.2016.12.016
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发表时间：
2017-02-28
影响因子：
3.4
通讯作者：
Wax, Adam
中科院分区：
文献类型：
--
作者：
Eldridge, Will J.;Steelman, Zachary A.;Wax, Adam
细胞生物学家一直在努力了解细胞对机械刺激的反应机制。为此，已经开发了许多流变学工具来表征细胞的硬度。然而，大多数微观流变学方法的性质限制了对细胞粘弹性性质的测量，这些方法需要在单细胞水平上进行直接机械接触。在这篇文章中，我们描述了一种新的定量相位成像分析方法，它将折射率变化与无序强度联系起来，无序强度是与细胞硬度有关的参数。值得注意的是，无序强度和细胞硬度都是用相同的相位成像系统测量的，这为细胞流变特性的无标记、非接触、单次成像提供了一种独特的替代方案。为了证明这项技术的潜在适用性，我们测量了具有不同力学性质的五个细胞群体的相序强度和剪切刚度，并证明了这两个参数之间的反向关系。这种关系的存在表明，可以通过使用这种据我们所知的新的非接触技术来检测细胞结构的无序强度来预测细胞的机械性能。
There have been sustained efforts on the part of cell biologists to understand the mechanisms by which cells respond to mechanical stimuli. To this end, many rheological tools have been developed to characterize cellular stiffness. However, measurement of cellular viscoelastic properties has been limited in scope by the nature of most microrheological methods, which require direct mechanical contact, applied at the single-cell level. In this article, we describe, to our knowledge, a new analysis approach for quantitative phase imaging that relates refractive index variance to disorder strength, a parameter that is linked to cell stiffness. Significantly, both disorder strength and cell stiffness are measured with the same phase imaging system, presenting a unique alternative for label-free, noncontact, single-shot imaging of cellular rheologic properties. To demonstrate the potential applicability of the technique, we measure phase disorder strength and shear stiffness across five cellular populations with varying mechanical properties and demonstrate an inverse relationship between these two parameters. The existence of this relationship suggests that predictions of cell, mechanical properties can be obtained from examining the disorder strength of cell structure using this, to our knowledge, novel, noncontact technique.