The grimace scale reliably assesses chronic pain in a rodent model of trigeminal neuropathic pain.
The grimace scale reliably assesses chronic pain in a rodent model of trigeminal neuropathic pain.
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DOI：
10.1016/j.ynpai.2017.10.001
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发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Keller A
中科院分区：
文献类型：
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作者：
Akintola T;Raver C;Studlack P;Uddin O;Masri R;Keller A
观察眶下神经缩窄性损伤后面部表情变化。在该模型中，鬼脸评分可靠地评估了持续疼痛。鬼脸评分可用于三叉神经病理性疼痛的大鼠和小鼠。将基础科学发现转化为有效的疼痛管理疗法的有限成功部分反映了可靠评估实验动物疼痛的困难。这种缺点在慢性持续疼痛领域尤其严重。面部表情的定量分析-鬼脸评分-被认为是一种有前途的工具，然而，它被认为只能可靠地评估短期或中期（几分钟到几小时）的疼痛。在这里，我们测试的假设，鬼脸评分是一个可靠的度量正在进行的神经性疼痛，通过测试的预测，慢性压迫性损伤的眶下神经（CCI-ION）将引起显着增加鬼脸量表评分。小鼠和大鼠接受CCI-ION，并在手术后10天或更长时间内测试机械超敏反应和鬼脸评分的变化。与假手术动物相比，CCI-ION大鼠和小鼠的鬼脸评分显著更高，从施加于面部的机械刺激中退缩的阈值显著更低。芬太尼逆转了大鼠鬼脸量表评分的变化，表明这些评分反映了疼痛感知。这些发现验证了鬼脸量表作为评估慢性三叉神经病理性疼痛啮齿动物模型中持续疼痛的可靠和敏感的度量。
Facial expressions were analyzed after constriction injury of infraorbital nerve. The grimace score reliably assesses ongoing pain in a this model. The grimace score can be used in both rats and mice with trigeminal neuropathic pain. The limited success in translating basic science findings into effective pain management therapies reflects, in part, the difficulty in reliably assessing pain in experimental animals. This shortcoming is particularly acute in the field of chronic, ongoing pain. Quantitative analysis of facial expressions—the grimace score—was introduced as a promising tool, however, it is thought to reliably assess only pain of short or medium duration (minutes to hours). Here, we test the hypothesis that grimace scores are a reliable metric of ongoing neuropathic pain, by testing the prediction that chronic constriction injury of the infraorbital nerve (CCI-ION) will evoke significant increases in grimace scale scores. Mice and rats were subjected to CCI-ION, and tested for changes in mechanical hypersensitivity and in grimace scores, 10 or more days after surgery. Both rats and mice with CCI-ION had significantly higher grimace scores, and significantly lower thresholds for withdrawal from mechanical stimuli applied to the face, compared to sham-operated animals. Fentanyl reversed the changes in rat grimace scale scores, suggesting that these scores reflect pain perception. These findings validate the grimace scale as a reliable and sensitive metric for the assessment of ongoing pain in a rodent model of chronic, trigeminal neuropathic pain.