Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells
Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells
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库普弗细胞表面受体CD16a对肝癌细胞生长的影响
DOI：
10.3892/ijmm.2016.2561
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发表时间：
2016-06-01
影响因子：
5.4
通讯作者：
Ding, Xiong
中科院分区：
文献类型：
--
作者：
Li, Xiu-Yun;Wu, Lun;Ding, Xiong
FcRIIIa (CD16) is a low-affinity Fc receptor of IgG. As the idio-binding receptor of IgG Fc, it plays an important role in the antibody-dependent cellular cytotoxicity of natural killer cells. The aim of the present study was to investigate the distribution of Kupffer cells (KCs) and the expression of their surface receptor FcRIIIa in hepatocellular carcinoma. Furthermore, we also aimed to observe the functional mechanism of FcRIIIa. Immunohistochemical analysis was employed to study KCs and FcRIIIa. In order to explore the role of FcRIIIa in the growth of cancer cells, KCs and H22 tumor cells were co-cultured in different serum. The mRNA expression levels of tumor necrosis factor (TNF)- and FcRIIIa were analyzed by RT-qPCR; the TNF- and FcRIIIa protein expression levels were examined by enzyme-linked immunosorbent assay and western blot analysis, respectively. Our results showed that the number of Kuppfer cells in cancerous tissues (21.6 +/- 7.8) was lower than those in para-cancerous (68.8 +/- 9.1) tissues and adjacent normal hepatic tissues (62.0 +/- 1.9) (P
