FgPal1 regulates morphogenesis and pathogenesis in Fusarium graminearum.
FgPal1 regulates morphogenesis and pathogenesis in Fusarium graminearum.
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DOI：
10.1111/1462-2920.15266
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发表时间：
2020-09
影响因子：
5.1
通讯作者：
Jinrong Yin;Chaofeng Hao;Gang-gang Niu;Wei Wang;Guanghui Wang;Ping Xiang;Jin-Rong Xu;Xue Zhang
中科院分区：
文献类型：
--
作者：
Jinrong Yin;Chaofeng Hao;Gang-gang Niu;Wei Wang;Guanghui Wang;Ping Xiang;Jin-Rong Xu;Xue Zhang
子囊孢子是小麦赤霉病病原菌--禾谷镰刀菌的主要接菌。在先前的研究中，发现FgPAL1在FGama1突变体中上调，对子囊孢子的发生很重要。然而，这个保守的基因在丝状子囊菌中的生物学功能尚不清楚。在这项研究中，我们描述了它在生长、分化和发病机制中的功能。Fgpal1突变体有严重的生长缺陷，经常表现出异常的菌丝尖端。在穗轴组织中的侵染生长和在小麦穗中的传播存在缺陷。与野生型相比，Fgpal1突变体产生的分生孢子具有更少的隔膜和更多的核。在生长活跃的菌丝顶端，FgPal1-GFP主要定位于近顶端和隔膜。FgPal1和LifeAct以相互依赖的方式部分共定位于心尖下区域。Fgpal1突变体减数分裂正常，发生ASCI时有8个核，但大多数ASCI都流产了。综上所述，我们的结果表明，FgPal1在维持两极顶端的生长以及核分裂和胞质分裂之间的协调方面发挥着重要作用，它还通过自由细胞形成过程对侵染性生长和子囊孢子的发育起着重要作用。这篇文章受版权保护。版权所有。
Ascospores are the primary inoculum in Fusarium graminearum, a causal agent of wheat head blight. In a previous study, FgPAL1 was found to be up-regulated in the Fgama1 mutant and important for ascosporogenesis. However, the biological function of this well-conserved gene in filamentous ascomycetes is not clear. In this study, we characterized its functions in growth, differentiation, and pathogenesis. The Fgpal1 mutant had severe growth defects and often displayed abnormal hyphal tips. It was defective in infectious growth in rachis tissues and spreading in wheat heads. The Fgpal1 mutant produced conidia with fewer septa and more nuclei per compartment than the wild type. In actively growing hyphal tips, FgPal1-GFP mainly localized to the subapical collar and septa. The FgPal1 and LifeAct partially co-localized at the subapical region in the interdependent manner. The Fgpal1 mutant was normal in meiosis with eight nuclei in developing asci but most asci were aborted. Taken together, our results showed that FgPal1 plays a role in maintaining polarized tip growth and coordination between nuclear division and cytokinesis, and it is also important for infectious growth and developments of ascospores by the free cell formation process. This article is protected by copyright. All rights reserved.