Establishment and assessments of a new model for the postoperative fatigue syndrome by major small intestinal resection in rats
Establishment and assessments of a new model for the postoperative fatigue syndrome by major small intestinal resection in rats
复制标题
大肠切除大鼠术后疲劳综合征新模型的建立与评估
DOI：
10.3109/00365521.2011.610001
复制
发表时间：
2011-11-01
影响因子：
1.9
通讯作者：
Yu, Zhen
中科院分区：
文献类型：
--
作者：
Zhang, Xiao-Dong;Chen, Bi-Cheng;Yu, Zhen
摘要目的。术后疲劳综合征（POFS）是手术后常见的主要并发症。然而，目前还没有稳定和标准化的POFS动物模型。本研究的目的是建立经小肠切除的POFS啮齿动物模型，并采用公认的物理和行为方法评估POFS。材料和方法。42只Sprague-Dawley大鼠根据“中段”小肠切除长度随机分为4组：0%（假手术组，即单纯开腹）、10%、40%、70%组，各取相应长度的小肠切除。手术后，对患者的总体健康状况进行评估。采用悬尾试验、野外开阔试验和Morris水迷宫试验评价POFS程度。测定血清白蛋白、转铁蛋白、前白蛋白和纤维连接蛋白以评估营养状况，同时测定超氧化物歧化酶（SOD）和丙二醛（MDA）。结果。与其他三组相比，70%小肠切除术组术后总体健康状况最差，悬尾试验强度下降，Morris水迷宫试验得分下降（p < 0.05）。切除小肠的大鼠均表现出一定程度的营养不良和抑郁行为，70%切除组转铁蛋白、前白蛋白和纤维连接蛋白水平较其他组最低（p < 0.05），血清SOD降低，MDA升高（p < 0.05）。结论。切除70%的小肠导致典型的POFS特征。该方法简单、稳定、可重复性好，可作为POFS研究的模型。
Abstract Objective. Postoperative fatigue syndrome (POFS) is a general and main complication after surgery. However, there is no stable and standardized animal model for POFS. The aim of the present study was to establish a rodent model of POFS by small intestinal resection, with POFS evaluated by acknowledged physical and behavioral methods. Material and Methods. Forty-two Sprague-Dawley rats were randomly divided into four groups according to the length of a “middle” small intestinal resection: 0% (sham group; i.e., laparotomy alone), 10%, 40% and 70% groups, with corresponding lengths of small intestinal resections. Following surgery, the general state of health was evaluated. Tail suspension test, open field test and Morris water maze test were used to evaluate the degree of POFS. Serum albumin, transferrin, prealbumin and fibronectin were measured to assess the nutritional status, and superoxide dismutase (SOD) and malondialdehyde (MDA) were also measured. Results. As compared with the other three groups, the 70% small intestinal resection group showed the worst general state of health, decreased strength of the tail suspension test and decreased score of Morris water maze test (p < 0.05) after operation. All rats in whom the small intestinal resection was done demonstrated a certain degree of malnutrition and behavior of depression, and the 70% resection group had the lowest levels of transferrin, prealbumin and fibronectin as compared with the other groups (p < 0.05), as well as decreased SOD and increased MDA in serum (p < 0.05). Conclusions. Resection of 70% of the small intestine resulted in typical characteristics of POFS. As this procedure is simple, stable and easily reproducible, it may serve as a model for research on POFS.