Δ9-THC, 11-OH-Δ9-THC and Δ9-THCCOOH plasma or serum to whole blood concentrations distribution ratios in blood samples taken from living and dead people
Δ9-THC, 11-OH-Δ9-THC and Δ9-THCCOOH plasma or serum to whole blood concentrations distribution ratios in blood samples taken from living and dead people
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DOI：
10.1016/s0379-0738(01)00538-2
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发表时间：
2001-12-01
影响因子：
2.2
通讯作者：
Mangin, P
中科院分区：
文献类型：
--
作者：
Giroud, C;Ménétrey, A;Mangin, P
大麻的娱乐性使用和滥用在瑞士不断增加。在从涉嫌在药物影响下驾驶的司机身上采集的生物样本中，经常单独或与其他药物混合检测到大麻素。此外，在参与各种法医事件的人的血液标本中也经常发现它们，例如杀人、谋杀、强奸和工伤事故。为了评估大麻暴露对人类行为和绩效的影响，通常需要估计大麻使用的时间。为此目的，Huestis及其同事建立了两个数学模型。这些模型基于血浆中的大麻素浓度。由于在我们的实验室中很少有血浆样本可用于法医测定，因此通过这些模型从全血值评估大麻使用的时间间隔可能是有用的。使用来自全血值的这些模型的一个先决条件是大麻素的血浆与全血浓度分布比的知识。在这方面，从8名定期吸食大麻的志愿者的血浆和全血中测量Δ（9）-THC、11-OH-Δ（9）-THC和Δ（9）-THCCOOH浓度。大麻素水平还确定了“血清”和全血样本从六具尸体。发现血浆与全血分布比的值非常相似，其个体变异系数相对较低，表明血浆水平可根据全血浓度计算，考虑乘数1.6。死后获得的数据表明，大麻素在全血和“血清”之间的分布比从活人身上确定的值分散在更大的范围内，（血清/全血浓度比的平均值= 2.4）。因此，部分依赖于选择适当的血液样品，即血浆。当无法获得血浆时，可谨慎考虑全血值，并考虑乘数1.6，以根据血液值计算血浆浓度。在死亡后采集的血液样本的情况下，不建议使用这些模型来评估大麻使用时间。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
The recreational use and abuse of Cannabis is continuously increasing in Switzerland. Cannabinoids are very often detected alone or in combination with other drugs in biological samples taken from drivers suspected of driving under the influence of drugs. Moreover, they are also frequently found in blood specimens from people involved in various medico-legal events, e.g. muggings, murders, rapes and working accidents as well. In order to assess the influence of Cannabis exposure on man behavior and performances, it is often needed to estimate the time of Cannabis use. For that purpose two mathematical models have been set up by Huestis and coworkers. These models are based on cannabinoids concentrations in plasma. Because plasma samples are rarely available for forensic determinations in our laboratory, it could be useful to assess the time-laps since Cannabis use through these models from whole blood values. One prerequisite to the use of these models from whole blood values is the knowledge of the plasma to whole blood concentrations distribution ratios of cannabinoids. In this respect, the Delta (9)-THC, 11-OH-Delta (9)-THC and Delta (9)-THCCOOH concentrations were measured in plasma and whole blood taken from eight volunteers who smoke Cannabis on a regular basis. Cannabinoids levels were also determined in "serum" and whole blood samples taken from six corpses. The values of the plasma to whole blood distribution ratios were found to be very similar and their individual coefficient of variation relatively low suggesting that plasma levels could be calculated from whole blood concentrations taken into account a multiplying factor of 1.6. The data obtained postmortem suggest that the distribution of cannabinoids between whole blood and "serum" is scattered over a larger range of values than those determined from living people and that more cannabinoids (mean value of the serum/whole blood concentrations ratios = 2.4) can be recovered from the "serum" fraction, The successful use of the mathematical models of Huestis and coworkers may, therefore, rely in part upon the selection of the appropriate blood sample, i.e. plasma. When plasma is not available, whole blood values could be considered with some caution taken into account a multiplying factor of 1.6 to calculate plasma concentrations from blood values. In the case of blood samples taken after death, the use of these models to assess the time of Cannabis use is not recommended. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.