Molecular typing of fowl adenoviruses, isolated in Hungary recently, reveals high diversity
Molecular typing of fowl adenoviruses, isolated in Hungary recently, reveals high diversity
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DOI：
10.1016/j.vetmic.2013.09.025
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发表时间：
2013-12-27
影响因子：
3.3
通讯作者：
Benko, Maria
中科院分区：
文献类型：
--
作者：
Kajan, Gyozo L.;Kecskemeti, Sandor;Benko, Maria
对2006至2011年间在匈牙利东部地区获得的26株新近分离的禽腺病毒(FAdV)进行了分子分型。这些病毒是在原代鸡胚肝细胞培养上从鸡的身体上分离出来的，并被送往进行诊断调查。对这些分离物进行了聚合酶链式反应，以扩增出六邻体基因的片段。对扩增片段的序列分析显示，该国所有五种FAdV物种都存在，其中包括FAdV-B，这种病毒以前在匈牙利几乎没有发现。这三个被归类为FAdV-B的毒株可能代表了该物种中的一个新类型，因为它们与340株(FAdV-5型)的核苷酸序列同源性低于普遍接受的型内限制。在本研究中，FAdV-E(12株)和FAdV-D(7株)是大多数(63%)的毒株。其中4株是属于两种不同FAdV型的腺病毒株的混合物，在这4例中的3例中，这两种类型甚至属于两个不同的种。对病理结果的分析支持了某些FAdV类型与疾病状况之间的某些既定联系。仅与FAdV-1毒株有关的胃黏膜糜烂，与FAdV-D和-E毒株有关的包涵体肝炎。(C)2013爱思唯尔B.V.保留所有权利。
Molecular typing of 26 recent fowl adenovirus (FAdV) isolates obtained in Eastern Hungary between 2006 and 2011 was performed. The viruses were isolated on primary chicken embryo liver cell cultures from carcasses of chickens, sent for diagnostic investigation. The isolates were subjected to a PCR to amplify a fragment from the hexon gene. Sequence analysis of the amplicons revealed the presence of all the five FAdV species in the country, among them FAdV-B, which has been barely found in Hungary before. The three strains classified as FAdV-B might represent a novel type within this species, as their nucleotide sequence identity to strain 340 (type FAdV-5) is below the commonly accepted intratype limit. Most of the strains (63%) were classified into species FAdV-E (12 strains) and FAdV-D (7 strains) in the study. Four of the isolates proved to be the mixture of two adenovirus strains belonging to two different FAdV types, in three from these four cases, these two types even belonged to two different species. Analysing the pathological findings bolsters certain established connections between some FAdV types and disease conditions. Gizzard erosion was found in connection with FAdV-1 strains only, and inclusion body hepatitis with FAdV-D and -E strains. (C) 2013 Elsevier B.V. All rights reserved.