MOSQUITO MASS REARING TECHNOLOGY: A COLD-WATER VORTEX DEVICE FOR CONTINUOUS UNATTENDED SEPARATION OF ANOPHELES ARABIENSIS PUPAE FROM LARVAE
MOSQUITO MASS REARING TECHNOLOGY: A COLD-WATER VORTEX DEVICE FOR CONTINUOUS UNATTENDED SEPARATION OF ANOPHELES ARABIENSIS PUPAE FROM LARVAE
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DOI:
10.2987/10-6085.1
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发表时间:
2011-09-01
影响因子:
1
通讯作者:
Benedict, Mark Q.
中科院分区:
文献类型:
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作者:
Balestrino, Fabrizio;Gilles, Jeremie R. L.;Benedict, Mark Q.
In mass rearing of anopheline mosquitoes, pupae are usually separated from larvae on a daily basis to prevent unwanted adult emergence from trays. Depending on the device and species, 2 physical characteristics have most often been used for separation: buoyant density and size. In this report, we describe a system for continuous separation of Anopheles arabiensis larvae from pupae based on the natural difference in buoyant density and behavior between the 2 stages. We determined that temperatures 4-15 degrees C caused neither mortality nor reduction in likelihood of pupation or emergence. Separation improved as temperatures decreased down to 4 degrees C. We devised and demonstrated a 15 degrees C water vortex separator that we anticipate can process approximately 1 million larvae and pupae per hour with