Penetration of phospholipid monolayers by cardiotoxins.
Penetration of phospholipid monolayers by cardiotoxins.
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心脏毒素渗透磷脂单层。
DOI：
10.1021/bi00520a017
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
R. Verger
中科院分区：
文献类型：
--
作者：
P. Bougis;H. Rochat;G. Piéroni;R. Verger
采用单分子膜技术比较了4种莫桑比克眼镜蛇心脏毒素与不同磷脂的特异性相互作用。我们能够证明心脏毒素（10（-7）M）与中性和带负电荷的磷脂在非常高的表面压力（45达因/厘米）下的相互作用。在磷脂单层的存在下，心脏毒素的表面活性变得比在空气-水界面处观察到的大得多。相同毒液的神经毒素不能穿透磷脂膜，即使在低表面压力（15达因/厘米）。心脏毒素III的表观分子面积在其插入到一个带负电荷的磷脂膜进行了定量定义。作为膜的表面压力的函数，大约25达因/厘米，心脏毒素可以以两种不同的构型存在，“平坦”（1400 A2）或“沿边”（420 A2）。这一结果可以解释这类毒素的溶解活性。
The monomolecular film technique was used to compare the specific interaction of four cardiotoxins from Naja mossambica mossambica with different phospholipids. We were able to demonstrate the interaction of cardiotoxins (10(-7) M) with both neutral and negatively charged phospholipids up to very high surface pressures (45 dyn/cm). In the presence of a phospholipid monolayer, the surface activity of cardiotoxins became much greater than that observed at the air-water interface. Neurotoxins of the same venom do not penetrate a phospholipid film, even at low surface pressure (15 dyn/cm). The apparent molecular area of cardiotoxin III during its insertion into a negatively charged phospholipid film was quantitatively defined. As a function of surface pressure of the membrane around 25 dyn/cm, cardiotoxins may exist in two different configurations, "flat" (1400 A2) or "edgewise" (420 A2). This result could account for the lytic activity of this type of toxin.