Cometabolism of methyl tert-butyl ether by a new microbial consortium ERS
Cometabolism of methyl tert-butyl ether by a new microbial consortium ERS
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新型微生物群 ERS 对甲基叔丁基醚的共代谢
DOI：
10.1007/s11356-015-4211-6
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发表时间：
2015-07-01
影响因子：
5.8
通讯作者：
Yan, Wei
中科院分区：
文献类型：
--
作者：
Li, Shanshan;Li, Danni;Yan, Wei
甲基叔丁基醚（MTBE）向环境中的释放增加了全世界对MTBE污染的关注。本文从某油站附近的土壤样品中分离得到一个微生物群落，该微生物群落可以直接降解MTBE，但生物量产量和MTBE降解效率较低。进一步的研究表明，当在正辛烷作为共代谢底物上生长时，该联合体可以有效降解 MTBE。基于对参与该联盟的所选操作分类单元（OTU）进行系统发育分析的 16S rDNA 结果显示，其中一个 OTU 与恶臭假单胞菌 GPo1 相关，后者可以在正辛烷生长时通过共代谢降解 MTBE。这可能有助于解释为什么正辛烷可能是 MTBE 降解的最佳共代谢底物。此外，随着正辛烷的消耗，观察到 MTBE 的降解。对于使用或不使用正辛烷生长的细胞，观察到不同的 MTBE Ks 值，这表明在正辛烷或 MTBE 上生长的细胞中，不同的酶负责 MTBE 的氧化。我们讨论了这些结果对我们理解 MTBE 生物降解和共代谢的影响。
The release of methyl tert-butyl-ether (MTBE) into the environment has increased the worldwide concern about the pollution of MTBE. In this paper, a microbial consortium was isolated from the soil sample near an oil station, which can degrade MTBE directly with a low biomass yield and MTBE degrading efficiency. Further research has indicated that this consortium can degrade MTBE efficiently when grown on n-octane as the cometabolic substrate. The results of 16S rDNA based on phylogenetic analysis of the selected operating taxonomic units (OTUs) involved in the consortium revealed that one OTU was related to Pseudomonas putida GPo1, which could cometabolically degrade MTBE on the growth of n-octane. This may help explain why n-octane could be the optimal cometabolic substrate of the consortium for MTBE degradation. Furthermore, the degradation of MTBE was observed along with the consumption of n-octane. Different Ks values for MTBE were observed for cells grown with or without n-octane, suggesting that different enzymes are responsible for the oxidation of MTBE in cells grown on n-octane or MTBE. The results are discussed in terms of their impacts on our understanding of MTBE biodegradation and cometabolism.