Differential expression of mRNA for leptin receptor isoforms in the rat brain
Differential expression of mRNA for leptin receptor isoforms in the rat brain
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DOI：
10.1016/s0303-7207(97)00138-x
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发表时间：
1997-09-30
影响因子：
4.1
通讯作者：
vanderPloeg, LHT
中科院分区：
文献类型：
--
作者：
Guan, XM;Hess, JF;vanderPloeg, LHT
瘦素通过与其受体（OB-R）在大脑中的相互作用，在控制食物摄入和能量代谢中发挥重要作用。已经鉴定出OB-R的几种选择性剪接异构体。为了研究这些OB-R在大脑中的表达模式和潜在的生物学功能，我们采用原位杂交技术检测了编码OB-R亚型的mRNA的分布。与先前的研究一致，OB-R mRNA在下丘脑、丘脑和脉络膜丛中检测到强信号。此外，在梨状皮质、小脑颗粒细胞层和黑质等其他脑区也观察到强烈的信号。利用同型特异性探针，揭示了OB-Rs的差异表达模式：OB-Ra和OB-Rb在下丘脑大量表达，而OB-Rc和OB-Rf不表达，而OB-Ra、OB-Rc和OB-Rf在脉络膜丛中显著表达，而OB-Rb不表达。OB-Rb在下丘脑的优先表达支持其介导瘦素饱腹效应的作用。OB-Ra和OB-Rb在下丘脑的共同表达可能表明这两种亚型之间可能存在相互作用。最后，在许多其他脑区检测OB-R mRNA可能表明瘦素参与了其他尚未确定的生理功能。(C) 1997爱思唯尔科学爱尔兰有限公司
Leptin plays an important role in the control of food intake and energy metabolism by interacting with its receptor (OB-R) in the brain. Several alternatively spliced isoforms of OB-R have been identified. To study the expression patterns and the potential biological function of these OB-Rs in the brain, the distribution of mRNA encoding OB-R isoforms was examined by in situ hybridization. In agreement with previous studies, strong signals for OB-R mRNA were detected in the hypothalamus, thalamus and choroid plexus. In addition, intense signals were observed in several other brain areas including piriform cortex, granule cell layer of the cerebellum and substantia nigra. With isoform-specific probes, a differential expression pattern of OB-Rs was revealed: OB-Ra and OB-Rb, but not OB-Rc and OB-Rf, are abundantly expressed in the hypothalamus, whereas OB-Ra, OB-Rc and OB-RF, but not OB-Rb, are significantly expressed in the choroid plexus. The preferential expression of OB-Rb in the hypothalamus is in support of its role in mediating the satiety effect of leptin. The co-expression of OB-Ra with OB-Rb in the hypothalamus may suggest a possible interaction between the two isoforms. Finally, the detection of OB-R mRNA in a number of other brain regions may indicate the involvement of leptin in additional as yet undefined physiological functions. (C) 1997 Elsevier Science Ireland Ltd.