Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner
Protein Corona Influences Cellular Uptake of Gold Nanoparticles by Phagocytic and Nonphagocytic Cells in a Size-Dependent Manner
复制标题
蛋白电晕以大小依赖性方式影响吞噬细胞和非吞噬细胞对金纳米颗粒的细胞摄取
DOI：
10.1021/acsami.5b04290
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发表时间：
2015-09-23
影响因子：
9.5
通讯作者：
Ge, Cuicui
中科院分区：
文献类型：
--
作者：
Cheng, Xiaju;Tian, Xin;Ge, Cuicui
纳米生物的相互作用是设计用于生物医学应用的安全纳米材料的关键问题。最近的研究表明，决定纳米颗粒细胞相互作用（包括内吞途径和生物反应）的是纳米颗粒-蛋白质冠，而不是裸露的纳米颗粒。在这里，我们展示了蛋白电晕对不同细胞系中不同尺寸金纳米粒子的细胞摄取的影响。实验结果表明，蛋白电晕以粒径和细胞类型依赖性方式显着降低 Au NP 的内化。与非吞噬细胞对小尺寸Au NPs的抑制相比，蛋白冠对吞噬细胞对大尺寸Au NPs的摄取表现出更显着的抑制作用。内吞实验表明，不同的内吞途径可能导致蛋白冠在不同大小的金纳米粒子与不同细胞系的相互作用中发挥不同的作用。我们的研究结果可以为生物医学应用中纳米材料的合理设计提供有用的信息。
The interaction at nanobio is a critical issue in designing safe nanomaterials for biomedical applications. Recent studies have reported that it is nanoparticle-protein corona rather than bare nanoparticle that determines the nanoparticle cell interactions, including endocytic pathway and biological responses. Here, we demonstrate the effects of protein corona on cellular uptake of different sized gold nanoparticles in different cell lines. The experimental results show that protein corona significantly decreases the internalization of Au NPs in a particle size- and cell type-dependent manner. Protein corona exhibits much more significant inhibition on the uptake of large-sized Au NPs by phagocytic cell than that of small-sized Au NPs by nonphagocytic cell. The endocytosis experiment indicates that different endocytic pathways might be responsible for the differential roles of protein corona in the interaction of different sized Au NPs with different cell lines. Our findings can provide useful information for rational design of nanomaterials in biomedical application.