Transcriptome analysis of<i>Aurantiochytrium limacinum</i>under low salt conditions
Transcriptome analysis of<i>Aurantiochytrium limacinum</i>under low salt conditions
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低盐条件下<i>Aurantiochytrium limacinum</i>的转录组分析
DOI：
10.1093/jambio/lxac004
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发表时间：
2023
影响因子：
4
通讯作者：
Sawayama Shigeki
中科院分区：
文献类型：
--
作者：
Kubo Yuki;Morimoto Daichi;Kato Shuri;Shiroi Mai;Ohara Kanta;Higashine Tokuhiro;Mori Yuki;Yoshimi Toru;Takeuchi Masato;Sawayama Shigeki
橙色壶菌可以积累大量的ω-3多不饱和脂肪酸，特别是二十二碳六烯酸（DHA）。虽然盐度影响DHA含量，但它对A. limacinumis不完全understood理解.为了解决这个问题，我们研究了A.低渗胁迫下的limacinum。我们首先培养了A. limacinum分别在典型和低盐度下进行RNA测序。转录组分析显示，933个基因在低渗条件下表现出显着的表达变化，其中81.4%下调。引人注目的是，A. limacinum下调与聚酮合成和脂肪酸合成酶途径相关的基因，而上调β-氧化相关基因。根据这一点，DHA的生产显着降低低渗条件下，而抗氧化剂相关基因显着上调。考虑到脂肪酸的β-氧化产生能量和活性氧（ROS），我们的研究结果表明A. limacinum利用脂肪酸作为能量在低渗条件下生存，并使用抗氧化系统解毒ROS。
Aurantiochytrium limacinumcan accumulate high amounts of omega-3 polyunsaturated fatty acids, especially docosahexaenoic acid (DHA). Although salinity affects the DHA content, its impact on the metabolic pathway responsible for DHA production inA. limacinumis not completely understood. To address this issue, we investigated the transcriptional profile ofA. limacinumunder hypoosmotic stress. We first culturedA. limacinumunder typical and low salinity for RNA sequencing, respectively. Transcriptome analyses revealed that 933 genes exhibited significant changes in expression under hypoosmotic conditions, of which 81.4% were downregulated. Strikingly,A. limacinumdownregulated genes related to polyketide synthesis and fatty acid synthase pathways, while upregulating β-oxidation-related genes. In accordance with this, DHA production significantly decreased under hypoosmotic conditions, while antioxidant-related genes were significantly upregulated. Considering that β-oxidation of fatty acids generates energy and reactive oxygen species (ROS), our results suggest thatA. limacinumutilizes fatty acids for energy to survive under hypoosmotic conditions and detoxifies ROS using antioxidant systems.