AMINERGIC NEURONS IN THE BRAIN OF BLOWFLIES AND DROSOPHILA - DOPAMINE-IMMUNOREACTIVE AND TYROSINE HYDROXYLASE-IMMUNOREACTIVE NEURONS AND THEIR RELATIONSHIP WITH PUTATIVE HISTAMINERGIC NEURONS
AMINERGIC NEURONS IN THE BRAIN OF BLOWFLIES AND DROSOPHILA - DOPAMINE-IMMUNOREACTIVE AND TYROSINE HYDROXYLASE-IMMUNOREACTIVE NEURONS AND THEIR RELATIONSHIP WITH PUTATIVE HISTAMINERGIC NEURONS
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DOI：
10.1007/bf00318701
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发表时间：
1992-01-01
影响因子：
3.6
通讯作者：
ELEKES, K
中科院分区：
文献类型：
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作者：
NASSEL, DR;ELEKES, K
本文对红头丽蝇和地不容丽蝇与多巴胺（DA）、酪氨酸羟化酶（TH）和组胺（HA）抗血清反应的神经元的分布和形态进行了分析。 TH-免疫反应（THIR）和HA-免疫反应（HAIR）神经元也映射在果蝇。 抗DA和TH的抗血清特异性地标记了丽蝇中相同的神经元。 结果表明，在绿头苍蝇的脑和食管下神经节中，约有300个神经元呈DA免疫反应（DAIR）和THIR。 这些神经元大多数位于两侧集群，一些分布为双边对，和两个腹侧不成对的正中（VUM）神经元被认为是在食管下神经节。 免疫反应过程中发现，在所有的隔间的蘑菇体，除了肾盏，在所有部门的中央体复合体，在髓质，小叶和小叶板的视叶，并在非肾小球神经元的前脑，tritcerebrum和食管下神经节。 触角叶未见DA或TH免疫反应。 在果蝇中，用TH抗血清检测到与果蝇神经元同源的神经元。 在果蝇和果蝇中，18个HA免疫反应阳性神经元位于前脑，2个位于食管下神经节。 HAIR神经元广泛分支，但除小叶内的突起外，所有的HAIR突起均见于非肾小球神经元。 后脑和后脑没有毛发突起。 双标记实验表明，TH和HA免疫反应不共定位在任何神经元。 然而，在某些区域，这两种制度之间存在大量重叠。 所描述的广泛树枝状的胺能神经元的形态表明它们在脑和食管下神经节中具有调节功能。
The distribution and morphology of neurons reacting with antisera against dopamine (DA), tyrosine hydroxylase (TH) and histamine (HA) were analyzed in the blowflies Calliphora erythrocephala and Phormia terraenovae. TH-immunoreactive (THIR) and HA-immunoreactive (HAIR) neurons were also mapped in the fruitfly Drosophila melanogaster. The antisera against DA and TH specifically labeled the same neurons in the blowflies. About 300 neurons displayed DA immunoreactivity (DAIR) and THIR in the brain and subesophageal ganglion of the blowflies. Most of these neurons were located in bilateral clusters; some were distributed as bilateral pairs, and two ventral unpaired median (VUM) neurons were seen in the subesophageal ganglion. Immunoreactive processes were found in all compartments of the mushroom bodies except the calyces, in all divisions of the central body complex, in the medulla, lobula and lobula plate of the optic lobe, and in non-glomerular neuropil of protocerebrum, tritocerebrum and the subesophageal ganglion. No DA or TH immunoreactivity was seen in the antennal lobes. In Drosophila, neurons homologous to the blowfly neurons were detected with the TH antiserum. In Phormia and Drosophila, 18 HA-immunoreactive neurons were located in the protocerebrum and 2 in the subesophageal ganglion. The HAIR neurons arborized extensively, but except for processes in the lobula, all HAIR processes were seen in non-glomerular neuropil. The deuto- and tritocerebrum was devoid of HAIR processes. Double labeling experiments demonstrated that TH and HA immunoreactivity was not colocalized in any neuron. In some regions there was, however, substantial superposition between the two systems. The morphology of the extensively arborizing aminergic neurons described suggests that they have modulatory functions in the brain and subesophageal ganglion.